Detailed information of TA system TA169171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 7686..8205
• Replicon: plasmid pBP5067_P3
• Accession: NZ_CP059809
• Organism: Enterococcus faecium strain BP5067

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: HI834_RS14140
• Protein ID: WP_000588503.1
• Coordinates: 7948..8205 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: HI834_RS14135
• Protein ID: WP_000388479.1
• Coordinates: 7686..7955 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI834_RS14100	3257..3673	-	417	WP_001232859.1	hypothetical protein	-
HI834_RS14105	3676..3960	-	285	WP_000353844.1	hypothetical protein	-
HI834_RS14110	4305..4550	+	246	WP_002311817.1	hypothetical protein	-
HI834_RS14115	4852..4947	-	96	WP_106913803.1	type I toxin-antitoxin system Fst family toxin	-
HI834_RS14120	5673..6353	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI834_RS14125	6429..6995	+	567	Protein_8	hypothetical protein	-
HI834_RS14130	7376..7555	+	180	WP_001120991.1	hypothetical protein	-
HI834_RS14135	7686..7955	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HI834_RS14140	7948..8205	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
HI834_RS14145	8843..9430	+	588	Protein_12	hypothetical protein	-
HI834_RS14150	9585..10904	+	1320	WP_000202380.1	IS1380-like element IS1678 family transposase	-
HI834_RS14155	11108..11350	+	243	Protein_14	hypothetical protein	-
HI834_RS14160	11515..12129	-	615	WP_001261742.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16666	16666
	-	inside	IScluster/Tn	-	-	1657..10904	9247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T169171 WP_000588503.1 NZ_CP059809:7948-8205 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T169171 NZ_CP076290:c1348286-1347540 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT169171 WP_000388479.1 NZ_CP059809:7686-7955 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT169171 NZ_CP076290:c1347539-1347369 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7