Detailed information of TA system TA169170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 35004..36112
• Replicon: plasmid pBP5067_P2
• Accession: NZ_CP059808
• Organism: Enterococcus faecium strain BP5067

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: HI834_RS14060
• Protein ID: WP_000233000.1
• Coordinates: 35243..36112 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: HI834_RS14055
• Protein ID: WP_000205227.1
• Coordinates: 35004..35228 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI834_RS14025	30417..30698	-	282	WP_000353845.1	hypothetical protein	-
HI834_RS14030	31074..31265	-	192	Protein_38	DUF536 domain-containing protein	-
HI834_RS14035	31390..32070	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI834_RS14040	32584..32880	+	297	WP_002360685.1	hypothetical protein	-
HI834_RS14045	32881..33297	+	417	WP_000323438.1	recombinase	-
HI834_RS14050	33299..34861	+	1563	WP_000136908.1	recombinase family protein	-
HI834_RS14055	35004..35228	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
HI834_RS14060	35243..36112	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
HI834_RS14065	36093..36827	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
HI834_RS14070	36860..37768	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
HI834_RS14075	37765..38307	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
HI834_RS14080	38400..39194	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T169170 WP_000233000.1 NZ_CP059808:35243-36112 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T169170 NZ_CP076290:562993-563343 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT169170 WP_000205227.1 NZ_CP059808:35004-35228 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT169170 NZ_CP076290:562706-562987 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: R3JIN1