Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 9852..10459 Replicon plasmid pBP3378_P3
Accession NZ_CP059796
Organism Enterococcus faecium strain BP3378

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag HI838_RS15055 Protein ID WP_002321032.1
Coordinates 9852..10202 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag HI838_RS15060 Protein ID WP_002287514.1
Coordinates 10196..10459 (-) Length 88 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI838_RS15030 6138..6722 - 585 WP_002299575.1 recombinase family protein -
HI838_RS15035 6933..7136 - 204 WP_002299573.1 hypothetical protein -
HI838_RS15040 7146..7568 - 423 WP_002303116.1 hypothetical protein -
HI838_RS15045 7703..8875 - 1173 WP_000195429.1 IS256-like element IS256 family transposase -
HI838_RS15050 9214..9828 + 615 WP_010729795.1 recombinase family protein -
HI838_RS15055 9852..10202 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HI838_RS15060 10196..10459 - 264 WP_002287514.1 hypothetical protein Antitoxin
HI838_RS15065 10800..10979 - 180 WP_002305761.1 hypothetical protein -
HI838_RS15070 11360..12067 - 708 WP_002353391.1 hypothetical protein -
HI838_RS15075 12060..12497 - 438 WP_002296237.1 hypothetical protein -
HI838_RS15080 12512..12763 - 252 WP_002296238.1 hypothetical protein -
HI838_RS15085 12763..12969 - 207 WP_002296239.1 hypothetical protein -
HI838_RS15090 13410..13670 - 261 WP_002296240.1 hypothetical protein -
HI838_RS15095 14036..14521 - 486 WP_002296241.1 single-stranded DNA-binding protein -
HI838_RS15100 14524..15423 - 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / erm(B) - 1..43862 43862


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-111)

Antitoxin

(4-87)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T169165 WP_002321032.1 NZ_CP059796:c10202-9852 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T169165 NZ_CP076285:c3059760-3059353 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT169165 WP_002287514.1 NZ_CP059796:c10459-10196 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT169165 NZ_CP076285:c3060007-3059741 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829F5H8


Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References