Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 472121..472692 Replicon chromosome
Accession NZ_CP059784
Organism Enterococcus faecium strain BA17124

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag HI828_RS02235 Protein ID WP_002286801.1
Coordinates 472351..472692 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag HI828_RS02230 Protein ID WP_002323011.1
Coordinates 472121..472351 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI828_RS02200 467257..468585 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
HI828_RS02205 468607..469233 + 627 WP_002286816.1 cysteine hydrolase -
HI828_RS02210 469416..469997 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
HI828_RS02215 470729..471076 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
HI828_RS02220 471052..471291 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
HI828_RS02225 471496..471834 - 339 WP_002286804.1 hypothetical protein -
HI828_RS02230 472121..472351 + 231 WP_002323011.1 hypothetical protein Antitoxin
HI828_RS02235 472351..472692 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HI828_RS02240 473542..473727 + 186 WP_002304207.1 hypothetical protein -
HI828_RS02245 474232..474426 + 195 WP_002297028.1 hypothetical protein -
HI828_RS02250 474584..474866 + 283 Protein_446 transposase -
HI828_RS02255 475110..475940 - 831 WP_002286789.1 manganese catalase family protein -
HI828_RS02260 476148..477482 + 1335 WP_002303373.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T169158 WP_002286801.1 NZ_CP059784:472351-472692 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T169158 NZ_CP076285:c1410411-1410253 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT169158 WP_002323011.1 NZ_CP059784:472121-472351 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT169158 NZ_CP076285:1410040-1410218 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTAGTTAGTTTATATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTAGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References