Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 155977..157114 Replicon plasmid pA3895_P1
Accession NZ_CP059773
Organism Enterococcus faecium strain A3895

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag HI845_RS14230 Protein ID WP_002401483.1
Coordinates 155977..156840 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag HI845_RS14235 Protein ID WP_000301765.1
Coordinates 156842..157114 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI845_RS14200 151870..153360 - 1491 WP_002380753.1 primase C-terminal domain-containing protein -
HI845_RS14205 153755..153994 - 240 WP_002380754.1 helix-turn-helix domain-containing protein -
HI845_RS14210 154238..154411 - 174 WP_171902975.1 rRNA adenine methyltransferase -
HI845_RS14215 154356..155093 - 738 WP_001038790.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
HI845_RS14220 155218..155301 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
HI845_RS14225 155350..155433 - 84 Protein_159 peptide-binding protein -
HI845_RS14230 155977..156840 - 864 WP_002401483.1 zeta toxin family protein Toxin
HI845_RS14235 156842..157114 - 273 WP_000301765.1 antitoxin Antitoxin
HI845_RS14240 157131..157340 - 210 WP_000527317.1 peptide-binding protein -
HI845_RS14245 157417..158313 - 897 WP_118215883.1 ParA family protein -
HI845_RS14250 158416..158676 - 261 Protein_164 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
HI845_RS14255 158846..159583 - 738 WP_182481801.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
HI845_RS14260 159708..159803 - 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
HI845_RS14265 159852..160091 - 240 WP_000635249.1 peptide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') / erm(B) - 1..210114 210114
- inside IScluster/Tn aac(6')-aph(2'') / erm(B) - 147400..163461 16061


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-213)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32486.06 Da        Isoelectric Point: 6.9965

>T169155 WP_002401483.1 NZ_CP059773:c156840-155977 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T169155 NZ_CP076285:c453837-453295 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT169155 WP_000301765.1 NZ_CP059773:c157114-156842 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT169155 NZ_CP076285:c454132-453821 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References