Detailed information of TA system TA169155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 155977..157114
• Replicon: plasmid pA3895_P1
• Accession: NZ_CP059773
• Organism: Enterococcus faecium strain A3895

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: HI845_RS14230
• Protein ID: WP_002401483.1
• Coordinates: 155977..156840 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: HI845_RS14235
• Protein ID: WP_000301765.1
• Coordinates: 156842..157114 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI845_RS14200	151870..153360	-	1491	WP_002380753.1	primase C-terminal domain-containing protein	-
HI845_RS14205	153755..153994	-	240	WP_002380754.1	helix-turn-helix domain-containing protein	-
HI845_RS14210	154238..154411	-	174	WP_171902975.1	rRNA adenine methyltransferase	-
HI845_RS14215	154356..155093	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI845_RS14220	155218..155301	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
HI845_RS14225	155350..155433	-	84	Protein_159	peptide-binding protein	-
HI845_RS14230	155977..156840	-	864	WP_002401483.1	zeta toxin family protein	Toxin
HI845_RS14235	156842..157114	-	273	WP_000301765.1	antitoxin	Antitoxin
HI845_RS14240	157131..157340	-	210	WP_000527317.1	peptide-binding protein	-
HI845_RS14245	157417..158313	-	897	WP_118215883.1	ParA family protein	-
HI845_RS14250	158416..158676	-	261	Protein_164	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
HI845_RS14255	158846..159583	-	738	WP_182481801.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI845_RS14260	159708..159803	-	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
HI845_RS14265	159852..160091	-	240	WP_000635249.1	peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'') / erm(B)	-	1..210114	210114
	-	inside	IScluster/Tn	aac(6')-aph(2'') / erm(B)	-	147400..163461	16061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T169155 WP_002401483.1 NZ_CP059773:c156840-155977 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T169155 NZ_CP076285:c453837-453295 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT169155 WP_000301765.1 NZ_CP059773:c157114-156842 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT169155 NZ_CP076285:c454132-453821 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3