Detailed information of TA system TA169151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 20124..21232
• Replicon: plasmid pA11051_P2
• Accession: NZ_CP059766
• Organism: Enterococcus faecium strain A11051

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: HI861_RS14960
• Protein ID: WP_000233000.1
• Coordinates: 20363..21232 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: HI861_RS14955
• Protein ID: WP_000205227.1
• Coordinates: 20124..20348 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI861_RS14930	15832..15918	-	87	Protein_17	tetracycline resistance protein	-
HI861_RS14935	16510..17190	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI861_RS14940	17704..18000	+	297	WP_002360685.1	hypothetical protein	-
HI861_RS14945	18001..18417	+	417	WP_000323438.1	recombinase	-
HI861_RS14950	18419..19981	+	1563	WP_000136908.1	recombinase family protein	-
HI861_RS14955	20124..20348	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
HI861_RS14960	20363..21232	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
HI861_RS14965	21213..21947	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
HI861_RS14970	21980..22888	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
HI861_RS14975	22885..23427	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
HI861_RS14980	23520..24314	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
HI861_RS14985	24407..25087	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T169151 WP_000233000.1 NZ_CP059766:20363-21232 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T169151 NZ_CP076283:c42889-42359 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGCAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTAATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT169151 WP_000205227.1 NZ_CP059766:20124-20348 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT169151 NZ_CP076283:c43162-42893 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: R3JIN1