Detailed information of TA system TA169148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 18323..19460
• Replicon: plasmid pA11051_P1
• Accession: NZ_CP059765
• Organism: Enterococcus faecium strain A11051

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: Q54944
• Locus tag: HI861_RS13255
• Protein ID: WP_001284311.1
• Coordinates: 18323..19186 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: HI861_RS13260
• Protein ID: WP_000301765.1
• Coordinates: 19188..19460 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI861_RS13225	13895..15190	-	1296	WP_002297218.1	ISL3-like element ISEfa11 family transposase	-
HI861_RS13230	15435..15686	+	252	Protein_18	GNAT family N-acetyltransferase	-
HI861_RS13235	15779..16573	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
HI861_RS13240	16983..17066	+	84	Protein_20	peptide-binding protein	-
HI861_RS13245	17115..17198	+	84	WP_031929417.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
HI861_RS13250	17323..18060	+	738	WP_001038796.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI861_RS13255	18323..19186	-	864	WP_001284311.1	toxin zeta	Toxin
HI861_RS13260	19188..19460	-	273	WP_000301765.1	antitoxin	Antitoxin
HI861_RS13265	19477..19686	-	210	WP_000527318.1	peptide-binding protein	-
HI861_RS13270	19784..20065	-	282	WP_143343782.1	hypothetical protein	-
HI861_RS13275	20090..20770	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI861_RS13280	20848..21051	-	204	Protein_28	hypothetical protein	-
HI861_RS13285	21129..21671	-	543	WP_002302857.1	hypothetical protein	-
HI861_RS13290	21732..24051	-	2320	Protein_30	hypothetical protein	-
HI861_RS13295	24048..24413	-	366	Protein_31	type IV secretory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(L) / ant(6)-Ia / aph(3')-III / erm(B) / aac(6')-aph(2'')	-	1..294680	294680
	-	inside	IScluster/Tn	tet(L) / ant(6)-Ia / aph(3')-III / erm(B)	-	6177..25146	18969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32404.02 Da
• Isoelectric Point: 6.9964

>T169148 WP_001284311.1 NZ_CP059765:c19186-18323 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T169148 NZ_CP076282:4573765-4573938 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT169148 WP_000301765.1 NZ_CP059765:c19460-19188 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT169148 NZ_CP076282:4573441-4573737 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 1GVN
• PDB: 3Q8X

Antitoxin

• AlphaFold DB: A0A829F0A3