Detailed information of TA system TA169144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 464002..464573
• Replicon: chromosome
• Accession: NZ_CP059764
• Organism: Enterococcus faecium strain A11051

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HI861_RS02215
• Protein ID: WP_105468411.1
• Coordinates: 464232..464573 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: HI861_RS02210
• Protein ID: WP_002323011.1
• Coordinates: 464002..464232 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI861_RS02185	459483..460811	+	1329	WP_002327434.1	FAD-containing oxidoreductase	-
HI861_RS02190	460833..461459	+	627	WP_002352507.1	cysteine hydrolase	-
HI861_RS02195	461642..462223	+	582	WP_002327433.1	TetR/AcrR family transcriptional regulator	-
HI861_RS02200	462588..463163	+	576	WP_002352505.1	SOS response-associated peptidase family protein	-
HI861_RS02205	463368..463706	-	339	WP_002306002.1	hypothetical protein	-
HI861_RS02210	464002..464232	+	231	WP_002323011.1	hypothetical protein	Antitoxin
HI861_RS02215	464232..464573	+	342	WP_105468411.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HI861_RS02220	465423..465608	+	186	WP_002304207.1	hypothetical protein	-
HI861_RS02225	466113..466307	+	195	WP_002297028.1	hypothetical protein	-
HI861_RS02230	466465..466747	+	283	Protein_441	transposase	-
HI861_RS02235	466991..467821	-	831	WP_002286789.1	manganese catalase family protein	-
HI861_RS02240	468029..469363	+	1335	WP_002286788.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13355.73 Da
• Isoelectric Point: 10.2170

>T169144 WP_105468411.1 NZ_CP059764:464232-464573 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVIRPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T169144 NZ_CP076282:c3187235-3186828 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT169144 WP_002323011.1 NZ_CP059764:464002-464232 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT169144 NZ_CP076282:c3187482-3187216 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9