Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 33627..34764 Replicon plasmid pA10290_P2
Accession NZ_CP059757
Organism Enterococcus faecium strain A10290

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag HI839_RS15060 Protein ID WP_182482948.1
Coordinates 33901..34764 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag HI839_RS15055 Protein ID WP_000301765.1
Coordinates 33627..33899 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI839_RS15015 28983..29336 - 354 WP_002287659.1 IS66 family insertion sequence element accessory protein TnpB -
HI839_RS15020 29326..29520 - 195 WP_002285758.1 hypothetical protein -
HI839_RS15025 29603..30070 + 468 Protein_32 aminoglycoside phosphotransferase family protein -
HI839_RS15030 30200..31372 - 1173 WP_000195429.1 IS256-like element IS256 family transposase -
HI839_RS15035 31475..31873 + 399 Protein_34 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
HI839_RS15040 32043..32303 + 261 Protein_35 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
HI839_RS15045 32406..33302 + 897 WP_118215883.1 ParA family protein -
HI839_RS15050 33400..33609 + 210 WP_000527318.1 peptide-binding protein -
HI839_RS15055 33627..33899 + 273 WP_000301765.1 antitoxin Antitoxin
HI839_RS15060 33901..34764 + 864 WP_182482948.1 zeta toxin family protein Toxin
HI839_RS15065 35201..35518 + 318 WP_002300567.1 hypothetical protein -
HI839_RS15070 35557..36054 + 498 WP_169040278.1 molecular chaperone DnaJ -
HI839_RS15075 36144..36392 - 249 WP_032490935.1 hypothetical protein -
HI839_RS15080 36702..37280 + 579 WP_032490934.1 TetR/AcrR family transcriptional regulator -
HI839_RS15085 37312..37755 + 444 WP_032490933.1 small multi-drug export protein -
HI839_RS15090 37908..38093 + 186 WP_169040279.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid VanHAX - 1..74076 74076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32432.98 Da        Isoelectric Point: 6.6610

>T169142 WP_182482948.1 NZ_CP059757:33901-34764 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISAKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPIPPIPKTPKLPGL

Download         Length: 864 bp

>T169142 NZ_CP076282:c2727646-2727248 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT169142 WP_000301765.1 NZ_CP059757:33627-33899 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT169142 NZ_CP076282:c2727873-2727646 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References