Detailed information of TA system TA169142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 33627..34764
• Replicon: plasmid pA10290_P2
• Accession: NZ_CP059757
• Organism: Enterococcus faecium strain A10290

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: HI839_RS15060
• Protein ID: WP_182482948.1
• Coordinates: 33901..34764 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: HI839_RS15055
• Protein ID: WP_000301765.1
• Coordinates: 33627..33899 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI839_RS15015	28983..29336	-	354	WP_002287659.1	IS66 family insertion sequence element accessory protein TnpB	-
HI839_RS15020	29326..29520	-	195	WP_002285758.1	hypothetical protein	-
HI839_RS15025	29603..30070	+	468	Protein_32	aminoglycoside phosphotransferase family protein	-
HI839_RS15030	30200..31372	-	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
HI839_RS15035	31475..31873	+	399	Protein_34	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI839_RS15040	32043..32303	+	261	Protein_35	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
HI839_RS15045	32406..33302	+	897	WP_118215883.1	ParA family protein	-
HI839_RS15050	33400..33609	+	210	WP_000527318.1	peptide-binding protein	-
HI839_RS15055	33627..33899	+	273	WP_000301765.1	antitoxin	Antitoxin
HI839_RS15060	33901..34764	+	864	WP_182482948.1	zeta toxin family protein	Toxin
HI839_RS15065	35201..35518	+	318	WP_002300567.1	hypothetical protein	-
HI839_RS15070	35557..36054	+	498	WP_169040278.1	molecular chaperone DnaJ	-
HI839_RS15075	36144..36392	-	249	WP_032490935.1	hypothetical protein	-
HI839_RS15080	36702..37280	+	579	WP_032490934.1	TetR/AcrR family transcriptional regulator	-
HI839_RS15085	37312..37755	+	444	WP_032490933.1	small multi-drug export protein	-
HI839_RS15090	37908..38093	+	186	WP_169040279.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	VanHAX	-	1..74076	74076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32432.98 Da
• Isoelectric Point: 6.6610

>T169142 WP_182482948.1 NZ_CP059757:33901-34764 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISAKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPIPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T169142 NZ_CP076282:c2727646-2727248 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT169142 WP_000301765.1 NZ_CP059757:33627-33899 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT169142 NZ_CP076282:c2727873-2727646 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3