Detailed information of TA system TA169140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 50809..51328
• Replicon: plasmid pA10290_P1
• Accession: NZ_CP059756
• Organism: Enterococcus faecium strain A10290

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: HI839_RS13740
• Protein ID: WP_000588503.1
• Coordinates: 51071..51328 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: HI839_RS13735
• Protein ID: WP_000388479.1
• Coordinates: 50809..51078 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI839_RS13690	46006..46905	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HI839_RS13695	46908..47393	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
HI839_RS13700	47534..47737	-	204	WP_002316074.1	putative holin-like toxin	-
HI839_RS13705	47812..48072	+	261	WP_002295623.1	hypothetical protein	-
HI839_RS13710	48509..48715	+	207	WP_002347218.1	hypothetical protein	-
HI839_RS13715	48715..48966	+	252	WP_002296238.1	hypothetical protein	-
HI839_RS13720	48981..49418	+	438	WP_002300851.1	hypothetical protein	-
HI839_RS13725	49411..50118	+	708	WP_002303117.1	hypothetical protein	-
HI839_RS13730	50499..50678	+	180	WP_001120991.1	hypothetical protein	-
HI839_RS13735	50809..51078	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HI839_RS13740	51071..51328	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
HI839_RS13745	51787..51942	+	156	WP_002347142.1	hypothetical protein	-
HI839_RS13750	51966..52790	+	825	WP_001809248.1	hypothetical protein	-
HI839_RS13755	52955..53569	-	615	WP_001261742.1	recombinase family protein	-
HI839_RS13760	53807..54229	+	423	WP_002303116.1	hypothetical protein	-
HI839_RS13765	54239..54442	+	204	WP_002299573.1	hypothetical protein	-
HI839_RS13770	54653..55237	+	585	WP_002299575.1	recombinase family protein	-
HI839_RS13775	55426..56106	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(L) / ant(6)-Ia / aph(3')-III / erm(B)	-	1..246466	246466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T169140 WP_000588503.1 NZ_CP059756:51071-51328 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T169140 NZ_CP076282:c2225422-2225168 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT169140 WP_000388479.1 NZ_CP059756:50809-51078 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT169140 NZ_CP076282:c2225670-2225419 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7