Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 22150..22757 Replicon plasmid pA7214_P2
Accession NZ_CP059749
Organism Enterococcus faecium strain A7214

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag HI844_RS14900 Protein ID WP_002321032.1
Coordinates 22407..22757 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag HI844_RS14895 Protein ID WP_002287514.1
Coordinates 22150..22413 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI844_RS14870 18321..19550 + 1230 Protein_18 type I restriction-modification system subunit M -
HI844_RS14875 19605..20285 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
HI844_RS14880 20361..20549 + 189 Protein_20 hypothetical protein -
HI844_RS14885 20542..21249 + 708 WP_002353391.1 hypothetical protein -
HI844_RS14890 21630..21809 + 180 WP_002305761.1 hypothetical protein -
HI844_RS14895 22150..22413 + 264 WP_002287514.1 hypothetical protein Antitoxin
HI844_RS14900 22407..22757 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HI844_RS14905 22781..23395 - 615 WP_010729795.1 recombinase family protein -
HI844_RS14910 23845..25170 + 1326 WP_000969590.1 Y-family DNA polymerase -
HI844_RS14915 25163..25513 + 351 WP_000568378.1 hypothetical protein -
HI844_RS14920 25510..25689 + 180 WP_001021554.1 hypothetical protein -
HI844_RS14925 25825..26115 + 291 WP_000026576.1 hypothetical protein -
HI844_RS14930 26468..27271 + 804 WP_002311905.1 AAA family ATPase -
HI844_RS14935 27258..27584 + 327 WP_000796719.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX - 1..27932 27932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T169136 WP_002321032.1 NZ_CP059749:22407-22757 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T169136 NZ_CP076282:c1340608-1340501 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT169136 WP_002287514.1 NZ_CP059749:22150-22413 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT169136 NZ_CP076282:1340655-1340721 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References