Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 476700..477271 Replicon chromosome
Accession NZ_CP059747
Organism Enterococcus faecium strain A7214

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag HI844_RS02250 Protein ID WP_002286801.1
Coordinates 476930..477271 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag HI844_RS02245 Protein ID WP_002323011.1
Coordinates 476700..476930 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI844_RS02215 471959..473287 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
HI844_RS02220 473309..473935 + 627 WP_002286816.1 cysteine hydrolase -
HI844_RS02225 474118..474699 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
HI844_RS02230 475308..475655 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
HI844_RS02235 475631..475870 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
HI844_RS02240 476075..476413 - 339 WP_002286804.1 hypothetical protein -
HI844_RS02245 476700..476930 + 231 WP_002323011.1 hypothetical protein Antitoxin
HI844_RS02250 476930..477271 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HI844_RS02255 478121..478306 + 186 WP_002304207.1 hypothetical protein -
HI844_RS02260 478811..479005 + 195 WP_002297028.1 hypothetical protein -
HI844_RS02265 479163..479445 + 283 Protein_449 transposase -
HI844_RS02270 479689..480519 - 831 WP_002286789.1 manganese catalase family protein -
HI844_RS02275 480727..482061 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T169133 WP_002286801.1 NZ_CP059747:476930-477271 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T169133 NZ_CP076282:c490414-490196 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT169133 WP_002323011.1 NZ_CP059747:476700-476930 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT169133 NZ_CP076282:c490814-490440 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References