Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 17397..18534 Replicon plasmid pA4694_P4
Accession NZ_CP059743
Organism Enterococcus faecium strain A4694

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag HI848_RS14775 Protein ID WP_001284311.1
Coordinates 17671..18534 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag HI848_RS14770 Protein ID WP_000301765.1
Coordinates 17397..17669 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI848_RS14730 12509..12805 + 297 WP_000053907.1 replication control protein PrgN -
HI848_RS14735 12829..13788 - 960 WP_002287225.1 IS30 family transposase -
HI848_RS14740 14060..14740 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
HI848_RS14745 14815..15432 - 618 Protein_21 tetracycline resistance ribosomal protection protein -
HI848_RS14750 15523..16203 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
HI848_RS14755 16260..16760 + 501 Protein_23 IS6-like element IS1216 family transposase -
HI848_RS14760 16816..17073 + 258 WP_002347171.1 hypothetical protein -
HI848_RS14765 17171..17380 + 210 WP_000527318.1 peptide-binding protein -
HI848_RS14770 17397..17669 + 273 WP_000301765.1 antitoxin Antitoxin
HI848_RS14775 17671..18534 + 864 WP_001284311.1 toxin zeta Toxin
HI848_RS14780 18797..19534 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
HI848_RS14785 19659..19742 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
HI848_RS14790 19791..19874 - 84 Protein_30 peptide-binding protein -
HI848_RS14795 20031..20990 - 960 WP_002303350.1 IS30 family transposase -
HI848_RS14800 21350..22144 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
HI848_RS14805 22237..22488 - 252 Protein_33 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III - 1..24162 24162
- inside IScluster/Tn erm(B) / aph(3')-III - 3837..24028 20191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T169131 WP_001284311.1 NZ_CP059743:17671-18534 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T169131 NZ_CP076282:c278568-278290 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT169131 WP_000301765.1 NZ_CP059743:17397-17669 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT169131 NZ_CP076282:c278831-278571 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References