Detailed information of TA system TA169122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2166464..2167080
• Replicon: chromosome
• Accession: NZ_CP059738
• Organism: Pseudomonas sp. 29A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7H8GQU6
• Locus tag: H3303_RS09735
• Protein ID: WP_016987496.1
• Coordinates: 2166464..2166676 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7H8GRK7
• Locus tag: H3303_RS09740
• Protein ID: WP_016987497.1
• Coordinates: 2166676..2167080 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3303_RS09705	2162215..2162751	+	537	WP_007919961.1	DsbE family thiol:disulfide interchange protein	-
H3303_RS09710	2162748..2163218	+	471	WP_175554981.1	cytochrome c-type biogenesis protein CcmH	-
H3303_RS09715	2163215..2164417	+	1203	WP_175554980.1	c-type cytochrome biogenesis protein CcmI	-
H3303_RS09720	2164443..2164847	+	405	WP_016987493.1	hypothetical protein	-
H3303_RS09725	2164904..2165407	-	504	WP_026000781.1	PIN domain-containing protein	-
H3303_RS09730	2165489..2165956	-	468	WP_175554979.1	helix-turn-helix domain-containing protein	-
H3303_RS09735	2166464..2166676	+	213	WP_016987496.1	type II toxin-antitoxin system HicA family toxin	Toxin
H3303_RS09740	2166676..2167080	+	405	WP_016987497.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H3303_RS09745	2167147..2168334	-	1188	WP_016987498.1	MFS transporter	-
H3303_RS09750	2168578..2169198	+	621	WP_175554978.1	hypothetical protein	-
H3303_RS09755	2169441..2170439	-	999	WP_016987500.1	sulfate ABC transporter substrate-binding protein	-
H3303_RS09760	2170544..2171368	-	825	WP_038363916.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8059.19 Da
• Isoelectric Point: 10.1831

>T169122 WP_016987496.1 NZ_CP059738:2166464-2166676 [Pseudomonas sp. 29A]
VQSRQLIKELEEAGWVLDRVTGSHHIFRHRYNPYTIPVPHPKKDLPLGTVKSIRRRAGLYNPPASYEGDP

Nucleotide

• Length: 213 bp

>T169122 NZ_CP076279:3591559-3592023 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14788.77 Da
• Isoelectric Point: 4.4687

>AT169122 WP_016987497.1 NZ_CP059738:2166676-2167080 [Pseudomonas sp. 29A]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDSFEDAYKAAVEVAHIMLQEIAADGESIPMPTSASAHRSNPDFANMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT169122 NZ_CP076279:3591224-3591559 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7H8GQU6

Antitoxin

• AlphaFold DB: A0A7H8GRK7