Detailed information of TA system TA169121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2164904..2165956
• Replicon: chromosome
• Accession: NZ_CP059738
• Organism: Pseudomonas sp. 29A

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H3303_RS09725
• Protein ID: WP_026000781.1
• Coordinates: 2164904..2165407 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: H3303_RS09730
• Protein ID: WP_175554979.1
• Coordinates: 2165489..2165956 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3303_RS09700	2160230..2162218	+	1989	WP_175554982.1	heme lyase CcmF/NrfE family subunit	-
H3303_RS09705	2162215..2162751	+	537	WP_007919961.1	DsbE family thiol:disulfide interchange protein	-
H3303_RS09710	2162748..2163218	+	471	WP_175554981.1	cytochrome c-type biogenesis protein CcmH	-
H3303_RS09715	2163215..2164417	+	1203	WP_175554980.1	c-type cytochrome biogenesis protein CcmI	-
H3303_RS09720	2164443..2164847	+	405	WP_016987493.1	hypothetical protein	-
H3303_RS09725	2164904..2165407	-	504	WP_026000781.1	PIN domain-containing protein	Toxin
H3303_RS09730	2165489..2165956	-	468	WP_175554979.1	helix-turn-helix domain-containing protein	Antitoxin
H3303_RS09735	2166464..2166676	+	213	WP_016987496.1	type II toxin-antitoxin system HicA family toxin	-
H3303_RS09740	2166676..2167080	+	405	WP_016987497.1	type II toxin-antitoxin system HicB family antitoxin	-
H3303_RS09745	2167147..2168334	-	1188	WP_016987498.1	MFS transporter	-
H3303_RS09750	2168578..2169198	+	621	WP_175554978.1	hypothetical protein	-
H3303_RS09755	2169441..2170439	-	999	WP_016987500.1	sulfate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-91)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 19012.72 Da
• Isoelectric Point: 6.1005

>T169121 WP_026000781.1 NZ_CP059738:c2165407-2164904 [Pseudomonas sp. 29A]
MWLALSGRFRARWSKEIHSEWKRNLLKNRTDLTMEQLDRTSELMDRAIPEACVCDYEELVNGLSLPDQDDRHVLAAAIRC
GAGVIVTFNLKDFPDTQLAPFGFEAQHPDEFVENLFHLNSAAVVAAAQKQRKQLKTPAMDVESFLRLLQRQGLTQSVQAL
EGYRAIL

Nucleotide

• Length: 504 bp

>T169121 NZ_CP076279:c3322315-3321908 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17275.76 Da
• Isoelectric Point: 6.3819

>AT169121 WP_175554979.1 NZ_CP059738:c2165956-2165489 [Pseudomonas sp. 29A]
MSIVIHPPVTLPVEREIKAAIEGQRALAAYLATQFETQHIQIFDEQNEAHRVELPTSALRLLADILAALAEGNAVKVVPV
HAELTTQEAADLLNVSRPHMIKLLESGEISYHKTGKHRRVRFADLMDYKTRRDSASEQAMALLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT169121 NZ_CP076279:c3322562-3322296 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA