Detailed information of TA system TA169120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1841768..1842391
• Replicon: chromosome
• Accession: NZ_CP059738
• Organism: Pseudomonas sp. 29A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J3FUJ4
• Locus tag: H3303_RS08250
• Protein ID: WP_007911472.1
• Coordinates: 1842212..1842391 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: J2R7W3
• Locus tag: H3303_RS08245
• Protein ID: WP_007911473.1
• Coordinates: 1841768..1842175 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3303_RS08225	1837386..1838156	+	771	WP_175555118.1	trans-aconitate 2-methyltransferase	-
H3303_RS08230	1838302..1839045	-	744	WP_175555117.1	class I SAM-dependent methyltransferase	-
H3303_RS08235	1839610..1839972	+	363	WP_016982766.1	hypothetical protein	-
H3303_RS08240	1840190..1841404	-	1215	WP_016982765.1	aldose 1-epimerase family protein	-
H3303_RS08245	1841768..1842175	-	408	WP_007911473.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H3303_RS08250	1842212..1842391	-	180	WP_007911472.1	type II toxin-antitoxin system HicA family toxin	Toxin
H3303_RS08260	1842938..1843822	-	885	WP_175555116.1	alpha/beta hydrolase	-
H3303_RS08265	1844140..1844832	-	693	WP_175555115.1	pseudouridine synthase	-
H3303_RS08270	1844866..1845096	-	231	WP_038358305.1	cysteine-rich CWC family protein	-
H3303_RS08275	1845089..1846576	-	1488	WP_175555114.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6632.90 Da
• Isoelectric Point: 11.1923

>T169120 WP_007911472.1 NZ_CP059738:c1842391-1842212 [Pseudomonas sp. 29A]
VNSRFLIGQLVADGWYLVRIKGSHHHFKHPSKPGLVTVPHPKKDLLKKTAISILQQALL

Nucleotide

• Length: 180 bp

>T169120 NZ_CP076279:c3241323-3241015 [Escherichia coli]
ATGAAATTTCTACCTACAACAAATATGCGGGCGGCGAGGCCCTGCCTGTCGTCCGTGGCAGTCTGGCAAATGCTACTTAC
ACGACTGCTGGAACAGCATTATGGTCTGACACTAAACGATACACCTTTCTGTGATGAAACCGTCATACAGGAACATATCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATCTCTCACGATTATTGATATCATGAGGGCCGTCGCGATCTGGGTTTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14549.58 Da
• Isoelectric Point: 4.7324

>AT169120 WP_007911473.1 NZ_CP059738:c1842175-1841768 [Pseudomonas sp. 29A]
MLYPIAISIGDDHHAWGVEVPDIPGCFSAGEDLDDAVAMAREAIEGHFEILADDDAPIPAASKLSLHVANPKYAGCAWAV
VDIDVLKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT169120 NZ_CP076279:c3241700-3241344 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTGCCATACCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTGAAACGTGCTATGACACC
TTGTTTTGGAGCACGTCTGGTGCAGGAAGGCGACCGCCTGCATTTTCTCGCTGACCGCGCCGGGTTTAACGGTGCTTTCT
GCGACGATGACGCGCTGTACCTGGATCAGGCCTTTCCGTTGATTCTCAAACAACTGGAGCTGATACTCACCAGCGGAGAA
CTCTCCCCCCGCCATCAGCACTGTGTCACCCTCTATCACAACGGACTCACCTGCGAAGCTGATACGCTTGGTAGTTGTGG
CTACGTATACATCGCCATTTATCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: J3FUJ4

Antitoxin

• AlphaFold DB: J2R7W3