Detailed information of TA system TA169110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 6977871..6978274
• Replicon: chromosome
• Accession: NZ_CP059732
• Organism: Spirosoma sp. PL0136

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H3H32_RS29320
• Protein ID: WP_182464518.1
• Coordinates: 6977871..6978062 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H3H32_RS29325
• Protein ID: WP_182459271.1
• Coordinates: 6978059..6978274 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3H32_RS29300	6974978..6975349	+	372	WP_182459268.1	NADH-quinone oxidoreductase subunit A	-
H3H32_RS29305	6975416..6975961	+	546	WP_182459269.1	NADH-quinone oxidoreductase subunit B	-
H3H32_RS29310	6976043..6976543	+	501	WP_182459270.1	NADH-quinone oxidoreductase subunit C	-
H3H32_RS29315	6976630..6977862	+	1233	WP_157583963.1	NADH-quinone oxidoreductase subunit D	-
H3H32_RS29320	6977871..6978062	+	192	WP_182464518.1	type II toxin-antitoxin system HicA family toxin	Toxin
H3H32_RS29325	6978059..6978274	+	216	WP_182459271.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H3H32_RS29330	6978279..6978782	+	504	WP_182459272.1	NAD(P)H-dependent oxidoreductase subunit E	-
H3H32_RS29335	6978791..6980131	+	1341	WP_182459273.1	NADH-quinone oxidoreductase subunit NuoF	-
H3H32_RS29340	6980195..6981280	+	1086	WP_182459274.1	(2Fe-2S)-binding protein	-
H3H32_RS29345	6981377..6982531	+	1155	WP_182459275.1	NADH-quinone oxidoreductase subunit NuoH	-
H3H32_RS29350	6982585..6983151	+	567	WP_182459276.1	NADH-quinone oxidoreductase subunit I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-61)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-63)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7360.32 Da
• Isoelectric Point: 10.3476

>T169110 WP_182464518.1 NZ_CP059732:6977871-6978062 [Spirosoma sp. PL0136]
IKFREIVRLIEEDGWYQVRQRGSHRQFKHTSKTGLVTIAYHSLNDSVPSGTLSSIYKQAQIER

Nucleotide

• Length: 192 bp

>T169110 NZ_CP076279:c495064-494846 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7749.84 Da
• Isoelectric Point: 4.0086

>AT169110 WP_182459271.1 NZ_CP059732:6978059-6978274 [Spirosoma sp. PL0136]
MKKYLVIIEKSDTGYGAYVPDLPGCVATAPTKDEVERLIYEGIQFHIEGLELEGLSIPVASSEAETMVFAD

Nucleotide

• Length: 216 bp

>AT169110 NZ_CP076279:c495464-495090 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG