Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3709318..3709894 Replicon chromosome
Accession NZ_CP059723
Organism Escherichia coli strain BUCT2930

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H1R14_RS19590 Protein ID WP_001297643.1
Coordinates 3709318..3709605 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag H1R14_RS19595 Protein ID WP_000063160.1
Coordinates 3709592..3709894 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R14_RS19575 3705556..3706836 - 1281 WP_001037390.1 DUF445 domain-containing protein -
H1R14_RS19580 3706877..3708109 - 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
H1R14_RS19585 3708423..3709119 + 697 Protein_3485 IS1 family transposase -
H1R14_RS19590 3709318..3709605 + 288 WP_001297643.1 BrnT family toxin Toxin
H1R14_RS19595 3709592..3709894 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
H1R14_RS19600 3709928..3710884 - 957 WP_001297640.1 GTPase -
H1R14_RS19605 3710895..3711098 - 204 WP_000467859.1 YbdD/YjiX family protein -
H1R14_RS19610 3711148..3713298 - 2151 WP_001340937.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3708910..3709119 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T169104 WP_001297643.1 NZ_CP059723:3709318-3709605 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T169104 NZ_CP076277:c103875-103570 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCTTGCTGTCAGATAAAGTCTCCCGTGAACTTTATCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCTGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT169104 WP_000063160.1 NZ_CP059723:3709592-3709894 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT169104 NZ_CP076277:c104095-103877 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References