Detailed information of TA system TA169090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 712992..713518
• Replicon: chromosome
• Accession: NZ_CP059723
• Organism: Escherichia coli strain BUCT2930

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: H1R14_RS05220
• Protein ID: WP_000323025.1
• Coordinates: 712992..713279 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: H1R14_RS05225
• Protein ID: WP_000534858.1
• Coordinates: 713279..713518 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1R14_RS05175	708016..708231	-	216	WP_000839590.1	phage lysis protein EssD	-
H1R14_RS05180	708985..709200	-	216	WP_000066484.1	cold shock-like protein CspB	-
H1R14_RS05185	709501..709713	+	213	WP_000087756.1	cold shock-like protein CspF	-
H1R14_RS05190	709768..709857	+	90	WP_120795389.1	hypothetical protein	-
H1R14_RS05195	710135..710887	-	753	WP_061093371.1	antitermination protein	-
H1R14_RS05200	710901..711950	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
H1R14_RS05205	711952..712230	-	279	WP_012304870.1	hypothetical protein	-
H1R14_RS05210	712297..712548	-	252	WP_000980994.1	hypothetical protein	-
H1R14_RS05215	712765..712920	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
H1R14_RS05220	712992..713279	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
H1R14_RS05225	713279..713518	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
H1R14_RS05230	713543..713848	+	306	WP_001326990.1	hypothetical protein	-
H1R14_RS05235	714051..714383	+	333	WP_001301033.1	protein FlxA	-
H1R14_RS05240	714820..716133	-	1314	WP_000589005.1	ISNCY family transposase	-
H1R14_RS05245	717800..718156	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
H1R14_RS05250	718153..718404	-	252	Protein_698	DUF977 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	697563..727181	29618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T169090 WP_000323025.1 NZ_CP059723:c713279-712992 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T169090 NZ_CP076276:c2262389-2262135 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT169090 WP_000534858.1 NZ_CP059723:c713518-713279 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT169090 NZ_CP076276:c2262637-2262386 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8