Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 22489..24112 Replicon plasmid p1buct2930
Accession NZ_CP059722
Organism Escherichia coli strain BUCT2930

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag H1R14_RS00685 Protein ID WP_000088046.1
Coordinates 22489..23814 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag H1R14_RS00690 Protein ID WP_024136327.1
Coordinates 23819..24112 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R14_RS00655 19159..19458 - 300 WP_001572440.1 hypothetical protein -
H1R14_RS00660 19513..19749 - 237 Protein_24 hypothetical protein -
H1R14_RS00665 20203..20628 - 426 WP_001043843.1 hypothetical protein -
H1R14_RS00670 20882..21697 - 816 WP_001572439.1 HNH endonuclease -
H1R14_RS00675 21710..22120 - 411 WP_000985911.1 hypothetical protein -
H1R14_RS00680 22222..22428 - 207 WP_000134171.1 hypothetical protein -
H1R14_RS00685 22489..23814 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
H1R14_RS00690 23819..24112 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
H1R14_RS00695 24716..25108 - 393 WP_000341066.1 hypothetical protein -
H1R14_RS00700 25481..25822 + 342 WP_001151575.1 hypothetical protein -
H1R14_RS00705 25914..26105 + 192 WP_000398480.1 hypothetical protein -
H1R14_RS00710 26155..26436 + 282 WP_000077926.1 hypothetical protein -
H1R14_RS00715 26999..28240 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS2 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 - 1..218262 218262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T169087 WP_000088046.1 NZ_CP059722:c23814-22489 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T169087 NZ_CP076276:c1527129-1526935 [Escherichia coli]
ATGAGATATGACGACGTTAAACCATGCCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGGGATTTCAGGATATTACCG
TGTGAAATGCAATGGATGCGAATCACGATCTGGTTATGGAGGAAGTGAAAAAGCTGCCCTCGAAAGATGGAATAAACGAA
CCATTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT169087 WP_024136327.1 NZ_CP059722:c24112-23819 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT169087 NZ_CP076276:1526758-1526936 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTATAATTCATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTTCCTGA
GAGCATTTTTTCACATTCTGATTTGGTTAGTTTTGTTTTTGAGTATCTTGTCCAGTTGGTAGGCGAACCGCCTTCCTTTT
CAATAGTGGCAGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References