Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 229242..229768 Replicon plasmid pZHH-1
Accession NZ_CP059712
Organism Enterobacter hormaechei strain ZHH

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H2678_RS25085 Protein ID WP_000323025.1
Coordinates 229242..229529 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H2678_RS25090 Protein ID WP_000534858.1
Coordinates 229529..229768 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H2678_RS25050 224812..224988 - 177 WP_001371930.1 hypothetical protein -
H2678_RS25055 225499..226443 + 945 WP_000778029.1 DUF5417 domain-containing protein -
H2678_RS25060 226542..227141 + 600 WP_001031420.1 hypothetical protein -
H2678_RS25065 227201..227551 + 351 WP_000743059.1 hypothetical protein -
H2678_RS25070 227598..227801 + 204 WP_001015183.1 hypothetical protein -
H2678_RS25075 228083..228403 + 321 WP_000332796.1 hypothetical protein -
H2678_RS25080 229012..229170 - 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
H2678_RS25085 229242..229529 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H2678_RS25090 229529..229768 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H2678_RS25095 230031..230954 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
H2678_RS25100 231154..231726 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
H2678_RS25105 232202..233440 - 1239 WP_000219087.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / tet(A) / blaSHV-12 / dfrA16 / mcr-9 - 1..287277 287277


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T169083 WP_000323025.1 NZ_CP059712:c229529-229242 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T169083 NZ_CP076276:c501053-500835 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT169083 WP_000534858.1 NZ_CP059712:c229768-229529 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT169083 NZ_CP076276:c501453-501079 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References