Detailed information of TA system TA169069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2333375..2334114
• Replicon: chromosome
• Accession: NZ_CP059711
• Organism: Enterobacter hormaechei strain ZHH

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H2678_RS11315
• Protein ID: WP_038416064.1
• Coordinates: 2333375..2333860 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: H2678_RS11320
• Protein ID: WP_003857131.1
• Coordinates: 2333848..2334114 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2678_RS11295	2328730..2329170	-	441	WP_016239970.1	hypothetical protein	-
H2678_RS11300	2329323..2330684	-	1362	WP_000482601.1	FRG domain-containing protein	-
H2678_RS11305	2330698..2331501	-	804	WP_000589340.1	hypothetical protein	-
H2678_RS11310	2331560..2332906	-	1347	WP_076611902.1	ISNCY-like element ISLad2 family transposase	-
H2678_RS11315	2333375..2333860	-	486	WP_038416064.1	GNAT family N-acetyltransferase	Toxin
H2678_RS11320	2333848..2334114	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
H2678_RS11325	2334178..2335107	-	930	WP_038416065.1	LysR family transcriptional regulator	-
H2678_RS11330	2335237..2336625	+	1389	WP_038416066.1	MFS transporter	-
H2678_RS11335	2336604..2337161	-	558	WP_023300131.1	OmpH family outer membrane protein	-
H2678_RS11340	2337282..2338418	-	1137	WP_038416067.1	type 1 fimbrial protein	-
H2678_RS11345	2338442..2338966	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2314210..2334114	19904
	-	flank	IS/Tn	-	-	2331560..2332918	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17546.28 Da
• Isoelectric Point: 9.9308

>T169069 WP_038416064.1 NZ_CP059711:c2333860-2333375 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALAAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGKGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T169069 NZ_CP076274:c3960431-3960201 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT169069 WP_003857131.1 NZ_CP059711:c2334114-2333848 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT169069 NZ_CP076274:c3960204-3959845 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9