Detailed information of TA system TA169063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 906457..908084
• Replicon: chromosome
• Accession: NZ_CP059710
• Organism: Xanthomonas oryzae pv. oryzicola strain ML5

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: H4J00_RS04190
• Protein ID: WP_229689376.1
• Coordinates: 907524..908084 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: A0A5E8KSB4
• Locus tag: H4J00_RS04185
• Protein ID: WP_047340152.1
• Coordinates: 906457..907527 (-)
• Length: 357 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4J00_RS04165 (H4J00_04160)	902332..902658	-	327	WP_041183782.1	hypothetical protein	-
H4J00_RS04170 (H4J00_04165)	902962..904242	-	1281	WP_048485358.1	amidohydrolase family protein	-
H4J00_RS04175 (H4J00_04170)	904239..905648	-	1410	WP_047340153.1	amidohydrolase	-
H4J00_RS04180 (H4J00_04175)	905788..906348	+	561	WP_044750878.1	DUF924 family protein	-
H4J00_RS04185 (H4J00_04180)	906457..907527	-	1071	WP_047340152.1	macro domain-containing protein	Antitoxin
H4J00_RS04190 (H4J00_04185)	907524..908084	-	561	WP_229689376.1	DUF4433 domain-containing protein	Toxin
H4J00_RS04195 (H4J00_04190)	908508..909434	-	927	WP_024710586.1	Grx4 family monothiol glutaredoxin	-
H4J00_RS04200 (H4J00_04195)	909480..910271	+	792	WP_024710587.1	polysaccharide deacetylase family protein	-
H4J00_RS04210 (H4J00_04205)	910575..911246	-	672	WP_047340150.1	class I SAM-dependent methyltransferase	-
H4J00_RS04215 (H4J00_04210)	911569..912219	-	651	WP_024710589.1	SGNH/GDSL hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (5-185)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 21558.42 Da
• Isoelectric Point: 7.2479

>T169063 WP_229689376.1 NZ_CP059710:c908084-907524 [Xanthomonas oryzae pv. oryzicola]
MHCGNAAVQSSSWVSIGNPELIDKRSSHPVPLPPGGFSNDYVPFYFTPFLPMLRNIHTAWGGIQRRPNEDIVILVSSLHH
VAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHVPLDGLRGIVCHTDGL
QQTIERQLQERNLKLPVHTRTGWYFR

Nucleotide

• Length: 561 bp

>T169063 NZ_CP076274:c2851826-2851428 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 357 a.a.
• Molecular weight: 39970.97 Da
• Isoelectric Point: 6.0089

>AT169063 WP_047340152.1 NZ_CP059710:c907527-906457 [Xanthomonas oryzae pv. oryzicola]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDRADVRPLIEEVLAGLDTDILVFEPTLKYQNVAKRSG
VEKLTPARALIAELVRRYWVLGMECGLLEIQKLAWFLERNIERAGLPELDLRFSAHKYGPYADRLRHLLNGLDGSYLHCD
KRISDADPLDVIWFDDNRKSFVQTYLKSEAKQYIPALEATAALIDGFESPFGMELLATVDWLIVQEGISAQVADVREGIR
HWPGDQEAAARKDRLFDDRALGIALKRLASGAIAVA

Nucleotide

• Length: 1071 bp

>AT169063 NZ_CP076274:c2852053-2851826 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E8KSB4