Detailed information of TA system TA169049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3853885..3854630
• Replicon: chromosome
• Accession: NZ_CP059591
• Organism: Xanthomonas oryzae pv. oryzae strain IXO221

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO221_RS17960
• Protein ID: WP_205420989.1
• Coordinates: 3853885..3854214 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO221_RS17965
• Protein ID: WP_237384720.1
• Coordinates: 3854211..3854630 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO221_RS17930 (IXO221_017925)	3849015..3849686	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO221_RS17940 (IXO221_017935)	3849989..3850780	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO221_RS17945 (IXO221_017940)	3850826..3851752	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO221_RS17950 (IXO221_017945)	3852084..3852419	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
IXO221_RS17955 (IXO221_017950)	3852463..3853782	-	1320	WP_109182077.1	IS701-like element ISXo15 family transposase	-
IXO221_RS17960 (IXO221_017955)	3853885..3854214	+	330	WP_205420989.1	DUF4433 domain-containing protein	Toxin
IXO221_RS17965 (IXO221_017960)	3854211..3854630	+	420	WP_237384720.1	macro domain-containing protein	Antitoxin
IXO221_RS17970 (IXO221_017965)	3854682..3855650	+	969	WP_011258529.1	IS5-like element ISXo1 family transposase	-
IXO221_RS17975 (IXO221_017970)	3855842..3856810	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
IXO221_RS17980 (IXO221_017975)	3856963..3857601	+	639	WP_011407753.1	phosphate phosphatase	-
IXO221_RS17985 (IXO221_017980)	3857639..3858874	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
IXO221_RS17990 (IXO221_017985)	3859176..3859502	+	327	Protein_3445	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3852463..3858874	6411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13159.89 Da
• Isoelectric Point: 7.1161

>T169049 WP_205420989.1 NZ_CP059591:3853885-3854214 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSELSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T169049 NZ_CP076273:37339-37737 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGCACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15621.05 Da
• Isoelectric Point: 6.4733

>AT169049 WP_237384720.1 NZ_CP059591:3854211-3854630 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQLRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT169049 NZ_CP076273:37112-37339 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA