Detailed information of TA system TA169042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1254711..1256431
• Replicon: chromosome
• Accession: NZ_CP059590
• Organism: Xanthomonas oryzae pv. oryzae strain IXO685

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO685_RS05970
• Protein ID: WP_075244498.1
• Coordinates: 1254711..1255364 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO685_RS05975
• Protein ID: WP_075244497.1
• Coordinates: 1255361..1256431 (+)
• Length: 357 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO685_RS05945 (IXO685_0005970)	1250682..1251320	+	639	WP_011407760.1	SGNH/GDSL hydrolase family protein	-
IXO685_RS05950 (IXO685_0005975)	1251643..1252314	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO685_RS05960 (IXO685_0005985)	1252616..1253407	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO685_RS05965 (IXO685_0005990)	1253453..1254379	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO685_RS05970 (IXO685_0005995)	1254711..1255364	+	654	WP_075244498.1	DUF4433 domain-containing protein	Toxin
IXO685_RS05975 (IXO685_0006000)	1255361..1256431	+	1071	WP_075244497.1	macro domain-containing protein	Antitoxin
IXO685_RS05980 (IXO685_0006005)	1256469..1257704	-	1236	WP_263108882.1	ISL3-like element ISXoo13 family transposase	-
IXO685_RS05985 (IXO685_0006010)	1258006..1258332	+	327	Protein_1145	hypothetical protein	-
IXO685_RS05990 (IXO685_0006015)	1258693..1260294	+	1602	WP_011407751.1	rhamnogalacturonan lyase B N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1256469..1257704	1235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (16-216)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25229.65 Da
• Isoelectric Point: 8.3240

>T169042 WP_075244498.1 NZ_CP059590:1254711-1255364 [Xanthomonas oryzae pv. oryzae]
MAEHYSKLNPKNALIWRITHRGNLPWILDNGLHCGNAAVQSSSWVSIGNPELIDKRSSHAVPRPPGGFLNDYVPFYFTPF
SPMLHNIHTGWRGIPRRPNEDIVILVSSLHHVAERRWPFLFTDSHASCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDP
AKFERYQAEALIHRHLPLDGLRGIVCHTDGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 654 bp

>T169042 NZ_CP076272:3608967-3609431 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 357 a.a.
• Molecular weight: 39942.02 Da
• Isoelectric Point: 6.0080

>AT169042 WP_075244497.1 NZ_CP059590:1255361-1256431 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHLFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLAGLDTDILVFEPTLKYQNVAKRSG
VEKLTPARALIAELVRRYWVLGMECGLLEIQKLAWFLERNIERAGLPELDLRFSAHKYGPYADRLRHLLNGLDGSYLHCD
KRISDADPLDVIWFDDNRKSFVQTYLKSEAKQYIPALEATAALIDGFESPFGMELLATVDWLIVREGISAQVADVREGIR
HWPGGQEAAARKDRLFDDRALGIALKRLASGAIAVA

Nucleotide

• Length: 1071 bp

>AT169042 NZ_CP076272:3608632-3608967 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA