Detailed information of TA system TA169029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3906004..3906749
• Replicon: chromosome
• Accession: NZ_CP059587
• Organism: Xanthomonas oryzae pv. oryzae strain IXO644

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO644_RS18275
• Protein ID: WP_199285672.1
• Coordinates: 3906004..3906333 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO644_RS18280
• Protein ID: WP_011407754.1
• Coordinates: 3906330..3906749 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO644_RS18245 (IXO644_018210)	3901135..3901806	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO644_RS18255 (IXO644_018220)	3902108..3902899	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO644_RS18260 (IXO644_018225)	3902945..3903871	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO644_RS18265 (IXO644_018230)	3904203..3904538	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
IXO644_RS18270 (IXO644_018235)	3904582..3905901	-	1320	WP_155297135.1	IS701-like element ISXo15 family transposase	-
IXO644_RS18275 (IXO644_018240)	3906004..3906333	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
IXO644_RS18280 (IXO644_018245)	3906330..3906749	+	420	WP_011407754.1	macro domain-containing protein	Antitoxin
IXO644_RS18285 (IXO644_018250)	3906801..3907769	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
IXO644_RS18290 (IXO644_018255)	3907982..3908557	-	576	Protein_3503	IS701-like element ISXo15 family transposase	-
IXO644_RS18295 (IXO644_018260)	3909094..3909095	-	2	WP_099051302.1	IS5 family transposase	-
IXO644_RS18300 (IXO644_018265)	3909407..3909967	-	561	Protein_3505	IS701-like element ISXo15 family transposase	-
IXO644_RS18305 (IXO644_018270)	3910056..3911021	+	966	WP_109182027.1	IS1595-like element ISXo5 family transposase	-
IXO644_RS18310 (IXO644_018275)	3911028..3911219	-	192	Protein_3507	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3904582..3917153	12571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T169029 WP_199285672.1 NZ_CP059587:3906004-3906333 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T169029 NZ_CP076272:c547621-547403 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT169029 WP_011407754.1 NZ_CP059587:3906330-3906749 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT169029 NZ_CP076272:c548021-547647 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG