Detailed information of TA system TA169024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3850435..3851180
• Replicon: chromosome
• Accession: NZ_CP059586
• Organism: Xanthomonas oryzae pv. oryzae strain IXO639

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO639_RS17840
• Protein ID: WP_199285672.1
• Coordinates: 3850435..3850764 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO639_RS17845
• Protein ID: WP_237384720.1
• Coordinates: 3850761..3851180 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO639_RS17810 (IXO639_017860)	3845564..3846235	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO639_RS17820 (IXO639_017870)	3846538..3847329	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO639_RS17825 (IXO639_017875)	3847375..3848301	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO639_RS17830 (IXO639_017880)	3848633..3848968	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
IXO639_RS17835 (IXO639_017885)	3849012..3850332	-	1321	Protein_3414	IS701 family transposase	-
IXO639_RS17840 (IXO639_017890)	3850435..3850764	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
IXO639_RS17845 (IXO639_017895)	3850761..3851180	+	420	WP_237384720.1	macro domain-containing protein	Antitoxin
IXO639_RS17850 (IXO639_017900)	3851232..3852200	+	969	WP_011258529.1	IS5-like element ISXo1 family transposase	-
IXO639_RS17855 (IXO639_017905)	3852392..3853360	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
IXO639_RS17860 (IXO639_017910)	3853495..3854151	+	657	WP_075246166.1	phosphate phosphatase	-
IXO639_RS17865 (IXO639_017915)	3854189..3855405	-	1217	Protein_3420	ISL3 family transposase	-
IXO639_RS17870 (IXO639_017920)	3855707..3856033	+	327	Protein_3421	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3848857..3855405	6548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T169024 WP_199285672.1 NZ_CP059586:3850435-3850764 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T169024 NZ_CP076271:46996-47394 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15621.05 Da
• Isoelectric Point: 6.4733

>AT169024 WP_237384720.1 NZ_CP059586:3850761-3851180 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQLRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT169024 NZ_CP076271:46769-46996 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA