Detailed information of TA system TA169007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3896088..3896833
• Replicon: chromosome
• Accession: NZ_CP059582
• Organism: Xanthomonas oryzae pv. oryzae strain IXO220

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO220_RS18260
• Protein ID: WP_199285672.1
• Coordinates: 3896088..3896417 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO220_RS18265
• Protein ID: WP_237384720.1
• Coordinates: 3896414..3896833 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO220_RS18230 (IXO220_018260)	3891218..3891889	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO220_RS18240 (IXO220_018270)	3892192..3892983	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO220_RS18245 (IXO220_018275)	3893029..3893955	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO220_RS18250 (IXO220_018280)	3894287..3894622	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
IXO220_RS18255 (IXO220_018285)	3894666..3895985	-	1320	WP_109182077.1	IS701-like element ISXo15 family transposase	-
IXO220_RS18260 (IXO220_018290)	3896088..3896417	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
IXO220_RS18265 (IXO220_018295)	3896414..3896833	+	420	WP_237384720.1	macro domain-containing protein	Antitoxin
IXO220_RS18270 (IXO220_018300)	3896885..3897853	+	969	WP_011258529.1	IS5-like element ISXo1 family transposase	-
IXO220_RS18275 (IXO220_018305)	3898045..3899013	+	969	WP_011258188.1	IS5-like element ISXo1 family transposase	-
IXO220_RS18280 (IXO220_018310)	3899124..3899804	+	681	WP_011257926.1	phosphate phosphatase	-
IXO220_RS18285 (IXO220_018315)	3899842..3901058	-	1217	Protein_3505	ISL3 family transposase	-
IXO220_RS18290 (IXO220_018320)	3901360..3901686	+	327	Protein_3506	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3894666..3901058	6392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T169007 WP_199285672.1 NZ_CP059582:3896088-3896417 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T169007 NZ_CP076270:c1337765-1337658 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15621.05 Da
• Isoelectric Point: 6.4733

>AT169007 WP_237384720.1 NZ_CP059582:3896414-3896833 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQLRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT169007 NZ_CP076270:1337818-1337879 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT