Detailed information of TA system TA168999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1284348..1285093
• Replicon: chromosome
• Accession: NZ_CP059581
• Organism: Xanthomonas oryzae pv. oryzae strain IXO98

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO98_RS05890
• Protein ID: WP_199285672.1
• Coordinates: 1284348..1284677 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO98_RS05895
• Protein ID: WP_237384720.1
• Coordinates: 1284674..1285093 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO98_RS05860 (IXO98_005810)	1279477..1280148	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO98_RS05870 (IXO98_005820)	1280451..1281242	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO98_RS05875 (IXO98_005825)	1281288..1282214	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO98_RS05880 (IXO98_005830)	1282546..1282881	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
IXO98_RS05885 (IXO98_005835)	1282925..1284245	-	1321	Protein_1127	IS701 family transposase	-
IXO98_RS05890 (IXO98_005840)	1284348..1284677	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
IXO98_RS05895 (IXO98_005845)	1284674..1285093	+	420	WP_237384720.1	macro domain-containing protein	Antitoxin
IXO98_RS05900 (IXO98_005850)	1285145..1286113	+	969	WP_133265302.1	IS5-like element ISXo1 family transposase	-
IXO98_RS05905 (IXO98_005855)	1286305..1287273	+	969	WP_011258188.1	IS5-like element ISXo1 family transposase	-
IXO98_RS05910 (IXO98_005860)	1287384..1288064	+	681	WP_011257926.1	phosphate phosphatase	-
IXO98_RS05915 (IXO98_005865)	1288102..1289318	-	1217	Protein_1133	ISL3 family transposase	-
IXO98_RS05920 (IXO98_005870)	1289620..1289946	+	327	Protein_1134	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1282770..1289318	6548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T168999 WP_199285672.1 NZ_CP059581:1284348-1284677 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T168999 NZ_CP076268:4522789-4522962 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15621.05 Da
• Isoelectric Point: 6.4733

>AT168999 WP_237384720.1 NZ_CP059581:1284674-1285093 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQLRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT168999 NZ_CP076268:4522465-4522761 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG