Detailed information of TA system TA168975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1354014..1354600
• Replicon: chromosome
• Accession: NZ_CP059565
• Organism: Neisseria wadsworthii strain DSM 22245

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: G4CP80
• Locus tag: H3L96_RS06335
• Protein ID: WP_009116032.1
• Coordinates: 1354014..1354268 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G4CP81
• Locus tag: H3L96_RS06340
• Protein ID: WP_009116033.1
• Coordinates: 1354265..1354600 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3L96_RS06315	1349376..1349981	+	606	WP_182073114.1	trimeric intracellular cation channel family protein	-
H3L96_RS06320	1350005..1351498	-	1494	WP_182073115.1	ribonuclease G	-
H3L96_RS06325	1351884..1352660	+	777	WP_009116031.1	ferredoxin--NADP reductase	-
H3L96_RS06330	1352804..1353811	-	1008	WP_182073116.1	IS5 family transposase	-
H3L96_RS06335	1354014..1354268	+	255	WP_009116032.1	type II toxin-antitoxin system HicA family toxin	Toxin
H3L96_RS06340	1354265..1354600	+	336	WP_009116033.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H3L96_RS06345	1354766..1355227	+	462	WP_009116034.1	hypothetical protein	-
H3L96_RS06350	1355315..1357354	+	2040	WP_009116035.1	ATP-dependent DNA helicase RecG	-
H3L96_RS06355	1357544..1358821	+	1278	WP_009116037.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1352804..1353811	1007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9422.91 Da
• Isoelectric Point: 10.5624

>T168975 WP_009116032.1 NZ_CP059565:1354014-1354268 [Neisseria wadsworthii]
MKKKHQRTLALIFSRPVSGSIKWADIEALFIELGAEVQEREGSRIAVILFGQVKVFHRPHSSPDTDKGAVASVRKWLEEN
GVKP

Nucleotide

• Length: 255 bp

>T168975 NZ_CP076264:4343049-4343360 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12045.47 Da
• Isoelectric Point: 5.0956

>AT168975 WP_009116033.1 NZ_CP059565:1354265-1354600 [Neisseria wadsworthii]
MNNVMEINGHKAIIQYDPETELLRGEFIGLNGGADFYADNVADLHKEGAASLAVFLDVCKEKGIEPYKQYSGRFNVRVSP
ETHAAAAAAAAAQNISLNEWVNRAISQSAHI

Nucleotide

• Length: 336 bp

>AT168975 NZ_CP076264:4343361-4343651 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: G4CP80

Antitoxin

• AlphaFold DB: G4CP81