Detailed information of TA system TA168971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT-BrnA
• Location: 161133..161697
• Replicon: chromosome
• Accession: NZ_CP059564
• Organism: Alysiella filiformis strain DSM 16848

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H3L97_RS00930
• Protein ID: WP_097113825.1
• Coordinates: 161416..161697 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: H3L97_RS00925
• Protein ID: WP_097113724.1
• Coordinates: 161133..161429 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3L97_RS00900	156523..157008	+	486	WP_179655780.1	DNA starvation/stationary phase protection protein	-
H3L97_RS00905	157095..157802	-	708	WP_097113719.1	SAM-dependent methyltransferase	-
H3L97_RS00910	158082..159137	-	1056	WP_097113720.1	metallophosphoesterase	-
H3L97_RS00915	159219..159827	-	609	WP_097113721.1	DUF4230 domain-containing protein	-
H3L97_RS00920	160311..160985	-	675	WP_097113723.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-
H3L97_RS00925	161133..161429	-	297	WP_097113724.1	BrnA antitoxin family protein	Antitoxin
H3L97_RS00930	161416..161697	-	282	WP_097113825.1	BrnT family toxin	Toxin
H3L97_RS00935	161792..162238	-	447	WP_143269103.1	hypothetical protein	-
H3L97_RS00940	162268..162741	-	474	WP_097113726.1	preQ(1) synthase	-
H3L97_RS00945	162755..163009	-	255	WP_097113727.1	CopG family transcriptional regulator	-
H3L97_RS00950	162996..163262	-	267	WP_097113728.1	BrnT family toxin	-
H3L97_RS00955	163349..163951	-	603	WP_097113729.1	nicotinate (nicotinamide) nucleotide adenylyltransferase	-
H3L97_RS00960	163956..164483	-	528	WP_097113826.1	disulfide bond formation protein B	-
H3L97_RS00965	164691..164909	+	219	WP_179655782.1	NF038104 family lipoprotein	-
H3L97_RS00970	164902..165567	+	666	WP_097113732.1	7-cyano-7-deazaguanine synthase QueC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-86)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (34-91)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10910.06 Da
• Isoelectric Point: 5.1406

>T168971 WP_097113825.1 NZ_CP059564:c161697-161416 [Alysiella filiformis]
MIFEWDENKAESNFRKHGISFANAAKVFDDPNCITQQDRFENGEYRWQTIGMVGSEQLLLVAHTYLDDDENTVVRIISAR
KATKQERKNYDNH

Nucleotide

• Length: 282 bp

>T168971 NZ_CP076264:c3275963-3275556 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11374.94 Da
• Isoelectric Point: 5.1832

>AT168971 WP_097113724.1 NZ_CP059564:c161429-161133 [Alysiella filiformis]
MTIIRYKANEIPEPTAEDMARMAAIRDEDIDYSDIPPLTDEFWANAKRFNELYRPQKRQVTVRIDADVLAWLKQDGKGYQ
TRLNEILRQAMLSTKSAG

Nucleotide

• Length: 297 bp

>AT168971 NZ_CP076264:c3276210-3275944 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA