Detailed information of TA system TA168970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TfiT-relB/YafQ-RelB
• Location: 11547..12113
• Replicon: plasmid pDSM2580_1
• Accession: NZ_CP059561
• Organism: Conchiformibius steedae strain DSM 2580

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: -
• Locus tag: H3L98_RS00035
• Protein ID: WP_182078408.1
• Coordinates: 11547..11822 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H3L98_RS00040
• Protein ID: WP_182078415.1
• Coordinates: 11832..12113 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3L98_RS00015	7140..7856	-	717	WP_156932329.1	hypothetical protein	-
H3L98_RS00020	7924..8658	-	735	WP_182078412.1	DUF4261 domain-containing protein	-
H3L98_RS00025	9477..10673	-	1197	WP_182078413.1	plasmid recombination protein	-
H3L98_RS00030	11025..11339	+	315	WP_182078414.1	hypothetical protein	-
H3L98_RS00035	11547..11822	-	276	WP_182078408.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H3L98_RS00040	11832..12113	-	282	WP_182078415.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H3L98_RS00045	12848..13949	+	1102	Protein_8	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25740	25740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-88)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10704.39 Da
• Isoelectric Point: 6.8611

>T168970 WP_182078408.1 NZ_CP059561:c11822-11547 [Conchiformibius steedae]
MTAKYELAPTNQFKRDIKKRWAELLDSDWVTVANCLINDVELPIKYRDHELKGNHAGIRECHIKPDLLLMYEKQEQQIIL
IRIGTHSELFG

Nucleotide

• Length: 276 bp

>T168970 NZ_CP076264:c3139629-3139294 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10387.86 Da
• Isoelectric Point: 5.6479

>AT168970 WP_182078415.1 NZ_CP059561:c12113-11832 [Conchiformibius steedae]
MPSINYNIRLEQDLRNRAFAVFESYGLSPSQAIKLFLTQVADTHTIPLSFEHKANQEKIPNMATLAAIQEAKAGNLKGYA
SLDAMMDEIKQCD

Nucleotide

• Length: 282 bp

>AT168970 NZ_CP076264:c3139877-3139629 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA