Detailed information of TA system TA168967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 241098..242775
• Replicon: chromosome
• Accession: NZ_CP059560
• Organism: Aromatoleum petrolei strain ToN1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: ToN1_RS01055
• Protein ID: WP_169206412.1
• Coordinates: 241918..242775 (-)
• Length: 286 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ToN1_RS01050
• Protein ID: WP_169206411.1
• Coordinates: 241098..241925 (-)
• Length: 276 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ToN1_RS01030 (ToN1_02120)	236277..237779	-	1503	WP_169206408.1	aldehyde dehydrogenase	-
ToN1_RS01035 (ToN1_02130)	237788..238612	-	825	WP_210147955.1	aspartate dehydrogenase	-
ToN1_RS01040 (ToN1_02140)	238609..240294	-	1686	WP_169206409.1	thiamine pyrophosphate-binding protein	-
ToN1_RS01045 (ToN1_02150)	240284..241084	-	801	WP_169206410.1	SDR family oxidoreductase	-
ToN1_RS01050 (ToN1_02160)	241098..241925	-	828	WP_169206411.1	IclR family transcriptional regulator	Antitoxin
ToN1_RS01055 (ToN1_02170)	241918..242775	-	858	WP_169206412.1	alpha/beta hydrolase	Toxin
ToN1_RS01060 (ToN1_02180)	242791..243546	-	756	WP_210147956.1	SDR family oxidoreductase	-
ToN1_RS01065 (ToN1_02190)	243558..244331	-	774	WP_169206414.1	ABC transporter permease	-
ToN1_RS01070 (ToN1_02200)	244351..245343	-	993	WP_169206415.1	ABC transporter substrate-binding protein	-
ToN1_RS01075 (ToN1_02210)	245397..246266	-	870	WP_210147957.1	ABC transporter ATP-binding protein	-
ToN1_RS01080 (ToN1_02220)	246397..247260	-	864	WP_169206416.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (39-268)

Antitoxin

• Transcription regulator IclR, N-terminal (18-67)

Sequences

Toxin

Protein

• Length: 286 a.a.
• Molecular weight: 29783.99 Da
• Isoelectric Point: 6.4825

>T168967 WP_169206412.1 NZ_CP059560:c242775-241918 [Aromatoleum petrolei]
MRTHEERLAALGTHFPLVEAGEGGRRWTYRTAGRGGRTLVLLHGIGSSSASWLDVALAAGRDFRVVAWDAPGYGGSTPLA
AERPLATDYAQSLEGFLATLGIEGCVLVGHSLGALMATAYAAAYPRGRAAQLILVSPARGYGAREFDAERERVRASRLQA
LAALGVAGMAAERAGRLVSPSAGASVREWVRWNMASLNADGYRQATELLCGDDLARYAPAPLPVACFCGADDVVTPPAAC
EAVAAAYGVPLVLLPGCGHASPVEIPHLLAATLRESIEMKGECCE

Nucleotide

• Length: 858 bp

>T168967 NZ_CP076264:c2237441-2237187 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 276 a.a.
• Molecular weight: 30032.32 Da
• Isoelectric Point: 5.9732

>AT168967 WP_169206411.1 NZ_CP059560:c241925-241098 [Aromatoleum petrolei]
VSDDNSLDHPGNKYSVPALERGLRLLGEFSRHDPVLSAAELARRLDLPRSTVFRMLTTLEDLGFVERAEGGRDFRLGIAV
LRLGFEYLSSLELTELGMPILGRLRDEIGYASNLVVRDGRSIVYVAKVTPASWIASSVTVGTRLPAHATVFGRILLAELS
LDDLRALYPEEHLERHSGQTPATAMDLFNLLQLDKGRDCIVGEGFFEPSISTVAAPVRDREGQVIAALGATVPFGQIEEG
LRESLVTHIRAAALELSSALGYSQRRARTGKVISL

Nucleotide

• Length: 828 bp

>AT168967 NZ_CP076264:c2237689-2237438 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA