Detailed information of TA system TA168952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1347137..1348054
• Replicon: chromosome
• Accession: NZ_CP059497
• Organism: Bacillus velezensis strain JSRB 08

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: H2N97_RS06710
• Protein ID: WP_007407256.1
• Coordinates: 1347308..1348054 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: H2N97_RS06705
• Protein ID: WP_003154807.1
• Coordinates: 1347137..1347307 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2N97_RS06660	1342344..1342856	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
H2N97_RS06665	1342969..1343628	+	660	WP_017417606.1	terminase	-
H2N97_RS06670	1343526..1343993	+	468	Protein_1253	hypothetical protein	-
H2N97_RS06675	1344006..1344377	+	372	WP_017417608.1	hypothetical protein	-
H2N97_RS06680	1344382..1344579	+	198	WP_007610833.1	XkdX family protein	-
H2N97_RS06685	1344636..1345397	+	762	WP_017417609.1	hypothetical protein	-
H2N97_RS06690	1345448..1345711	+	264	WP_003154815.1	hemolysin XhlA family protein	-
H2N97_RS06695	1345725..1345988	+	264	WP_003154813.1	phage holin	-
H2N97_RS06700	1346002..1346880	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
H2N97_RS06705	1347137..1347307	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
H2N97_RS06710	1347308..1348054	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
H2N97_RS06715	1348158..1349156	-	999	WP_017417610.1	inorganic phosphate transporter	-
H2N97_RS06720	1349169..1349786	-	618	WP_003154804.1	DUF47 domain-containing protein	-
H2N97_RS06725	1350073..1351389	-	1317	WP_134986489.1	amino acid permease	-
H2N97_RS06730	1351711..1352661	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1306212..1406334	100122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T168952 WP_007407256.1 NZ_CP059497:c1348054-1347308 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T168952 NZ_CP076262:4360044-4360355 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT168952 WP_003154807.1 NZ_CP059497:c1347307-1347137 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT168952 NZ_CP076262:4360356-4360646 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14