Detailed information of TA system TA168950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1309068..1309985
• Replicon: chromosome
• Accession: NZ_CP059495
• Organism: Bacillus velezensis strain JSRB 166

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: H2N74_RS06875
• Protein ID: WP_007407256.1
• Coordinates: 1309239..1309985 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: H2N74_RS06870
• Protein ID: WP_003154807.1
• Coordinates: 1309068..1309238 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2N74_RS06835	1304280..1305869	+	1590	WP_029973066.1	hypothetical protein	-
H2N74_RS06840	1305882..1306307	+	426	WP_029973065.1	hypothetical protein	-
H2N74_RS06845	1306312..1306509	+	198	WP_007610833.1	XkdX family protein	-
H2N74_RS06850	1306566..1307327	+	762	WP_182072616.1	phage portal protein	-
H2N74_RS06855	1307379..1307642	+	264	WP_021493821.1	hemolysin XhlA family protein	-
H2N74_RS06860	1307656..1307919	+	264	WP_182072618.1	phage holin	-
H2N74_RS06865	1307933..1308811	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
H2N74_RS06870	1309068..1309238	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
H2N74_RS06875	1309239..1309985	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
H2N74_RS06880	1310089..1311087	-	999	WP_003154805.1	inorganic phosphate transporter	-
H2N74_RS06885	1311100..1311717	-	618	WP_003154804.1	DUF47 domain-containing protein	-
H2N74_RS06890	1312003..1313319	-	1317	WP_061862107.1	amino acid permease	-
H2N74_RS06895	1313642..1314592	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T168950 WP_007407256.1 NZ_CP059495:c1309985-1309239 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T168950 NZ_CP076262:3543743-3544207 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT168950 WP_003154807.1 NZ_CP059495:c1309238-1309068 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT168950 NZ_CP076262:3543408-3543743 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14