Detailed information of TA system TA168949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 483438..484075
• Replicon: chromosome
• Accession: NZ_CP059495
• Organism: Bacillus velezensis strain JSRB 166

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: H2N74_RS02425
• Protein ID: WP_003156187.1
• Coordinates: 483725..484075 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: H2N74_RS02420
• Protein ID: WP_003156188.1
• Coordinates: 483438..483719 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2N74_RS02400	479803..480402	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
H2N74_RS02405	480495..480860	+	366	WP_014304402.1	holo-ACP synthase	-
H2N74_RS02410	481025..482032	+	1008	WP_182072313.1	outer membrane lipoprotein carrier protein LolA	-
H2N74_RS02415	482149..483318	+	1170	WP_182072314.1	alanine racemase	-
H2N74_RS02420	483438..483719	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
H2N74_RS02425	483725..484075	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
H2N74_RS02430	484193..485014	+	822	WP_003156182.1	STAS domain-containing protein	-
H2N74_RS02435	485019..485384	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
H2N74_RS02440	485387..485788	+	402	WP_003156178.1	anti-sigma regulatory factor	-
H2N74_RS02445	485800..486807	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
H2N74_RS02450	486871..487200	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
H2N74_RS02455	487197..487679	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
H2N74_RS02460	487645..488433	+	789	WP_182072315.1	RNA polymerase sigma factor SigB	-
H2N74_RS02465	488433..489035	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T168949 WP_003156187.1 NZ_CP059495:483725-484075 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T168949 NZ_CP076262:c3277108-3276701 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT168949 WP_003156188.1 NZ_CP059495:483438-483719 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT168949 NZ_CP076262:c3277355-3277089 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5