Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2476488..2477124 Replicon chromosome
Accession NZ_CP059494
Organism Bacillus haynesii strain P19

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag H2R00_RS12250 Protein ID WP_003179128.1
Coordinates 2476488..2476838 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag H2R00_RS12255 Protein ID WP_006638778.1
Coordinates 2476843..2477124 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H2R00_RS12210 2471532..2472131 - 600 WP_182069236.1 SpoIIE family protein phosphatase -
H2R00_RS12215 2472128..2472919 - 792 WP_176277431.1 RNA polymerase sigma factor SigB -
H2R00_RS12220 2472885..2473367 - 483 WP_154999310.1 anti-sigma B factor RsbW -
H2R00_RS12225 2473367..2473693 - 327 WP_154999311.1 anti-sigma factor antagonist -
H2R00_RS12230 2473752..2474759 - 1008 WP_154999312.1 PP2C family protein-serine/threonine phosphatase -
H2R00_RS12235 2474770..2475171 - 402 WP_023857861.1 anti-sigma regulatory factor -
H2R00_RS12240 2475174..2475539 - 366 WP_043926799.1 STAS domain-containing protein -
H2R00_RS12245 2475543..2476370 - 828 WP_182069238.1 RsbT co-antagonist protein RsbRA -
H2R00_RS12250 2476488..2476838 - 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H2R00_RS12255 2476843..2477124 - 282 WP_006638778.1 hypothetical protein Antitoxin
H2R00_RS12260 2477236..2478405 - 1170 WP_182071070.1 alanine racemase -
H2R00_RS12265 2478631..2479590 - 960 WP_182071071.1 outer membrane lipoprotein carrier protein LolA -
H2R00_RS12270 2479934..2480533 + 600 WP_043926794.1 rhomboid family intramembrane serine protease -
H2R00_RS12275 2480530..2482014 - 1485 WP_182069240.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T168948 WP_003179128.1 NZ_CP059494:c2476838-2476488 [Bacillus haynesii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T168948 NZ_CP076262:c3134345-3134010 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT168948 WP_006638778.1 NZ_CP059494:c2477124-2476843 [Bacillus haynesii]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT168948 NZ_CP076262:c3134593-3134345 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References