Detailed information of TA system TA168943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2849842..2850503
• Replicon: chromosome
• Accession: NZ_CP059489
• Organism: Ralstonia solanacearum strain YQ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E7Z845
• Locus tag: H2F19_RS13160
• Protein ID: WP_016727753.1
• Coordinates: 2850090..2850503 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7Z6P3
• Locus tag: H2F19_RS13155
• Protein ID: WP_019717749.1
• Coordinates: 2849842..2850093 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2F19_RS13125	2845336..2845485	+	150	WP_155738951.1	hypothetical protein	-
H2F19_RS13130	2845546..2846160	-	615	WP_157698853.1	hypothetical protein	-
H2F19_RS13135	2846861..2847577	+	717	WP_049800424.1	Crp/Fnr family transcriptional regulator	-
H2F19_RS13140	2848252..2848467	-	216	WP_071624182.1	glycine zipper 2TM domain-containing protein	-
H2F19_RS13145	2848864..2849079	+	216	WP_071615547.1	hypothetical protein	-
H2F19_RS13150	2849233..2849465	-	233	Protein_2569	hypothetical protein	-
H2F19_RS13155	2849842..2850093	+	252	WP_019717749.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H2F19_RS13160	2850090..2850503	+	414	WP_016727753.1	type II toxin-antitoxin system VapC family toxin	Toxin
H2F19_RS13165	2850575..2851597	-	1023	WP_086706232.1	DNA cytosine methyltransferase	-
H2F19_RS13170	2851737..2852030	+	294	WP_020371973.1	hypothetical protein	-
H2F19_RS13175	2852418..2853263	+	846	WP_157698852.1	hypothetical protein	-
H2F19_RS13180	2853497..2854141	-	645	WP_086706230.1	hypothetical protein	-
H2F19_RS13185	2854217..2854369	-	153	WP_157698851.1	hypothetical protein	-
H2F19_RS13190	2854378..2854941	-	564	Protein_2577	hypothetical protein	-
H2F19_RS13195	2854894..2855181	+	288	WP_086706365.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2845954..2866606	20652
	-	flank	IS/Tn	-	-	2844374..2845339	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15586.89 Da
• Isoelectric Point: 6.2337

>T168943 WP_016727753.1 NZ_CP059489:2850090-2850503 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKAPDARVVAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDVQLINPWEA

Nucleotide

• Length: 414 bp

>T168943 NZ_CP076262:c1826578-1826291 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9305.49 Da
• Isoelectric Point: 5.0774

>AT168943 WP_019717749.1 NZ_CP059489:2849842-2850093 [Ralstonia solanacearum]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT168943 NZ_CP076262:c1826817-1826578 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA