Detailed information of TA system TA168941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2709627..2710223
• Replicon: chromosome
• Accession: NZ_CP059488
• Organism: Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: H2515_RS13555
• Protein ID: WP_198661480.1
• Coordinates: 2709627..2709920 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: H2515_RS13560
• Protein ID: WP_198660393.1
• Coordinates: 2709924..2710223 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2515_RS13530	2705069..2705539	+	471	WP_198660389.1	hypothetical protein	-
H2515_RS13535	2705774..2706100	+	327	Protein_2655	type II toxin-antitoxin system RelE/ParE family toxin	-
H2515_RS13540	2706187..2706633	+	447	WP_198660390.1	Hsp20/alpha crystallin family protein	-
H2515_RS13545	2707124..2708608	+	1485	WP_198660391.1	trypsin-like peptidase domain-containing protein	-
H2515_RS13550	2708977..2709468	+	492	WP_198660392.1	phosphate-starvation-inducible PsiE family protein	-
H2515_RS13555	2709627..2709920	+	294	WP_198661480.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2515_RS13560	2709924..2710223	+	300	WP_198660393.1	putative addiction module antidote protein	Antitoxin
H2515_RS13565	2711045..2712319	+	1275	WP_198660394.1	GGDEF domain-containing phosphodiesterase	-
H2515_RS13570	2712347..2713879	+	1533	WP_198660395.1	glycosyltransferase	-
H2515_RS13575	2713839..2714507	+	669	WP_198660396.1	PilZ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2701018..2710223	9205

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10997.75 Da
• Isoelectric Point: 10.0405

>T168941 WP_198661480.1 NZ_CP059488:2709627-2709920 [Acidithiobacillus ferrivorans]
ISIKTTAIFDRWLHGLRDRRARVRVQVRIDRMELAHFGDCEPVGEGVSEMRIHYGPGYRVYFKRFGLELVVLLAGGDKST
QSADIALALKLARQLEG

Nucleotide

• Length: 294 bp

>T168941 NZ_CP076262:c1506273-1506079 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGGGATTTCAGGATATTACCG
TGTGAAATGCAATGGATGCGAATCACGATCTGGTTATGGAGGAAGTGAAAAAGCTGCCCTCGAAAGATGGAATAAACGAA
CCATTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10698.17 Da
• Isoelectric Point: 6.0787

>AT168941 WP_198660393.1 NZ_CP059488:2709924-2710223 [Acidithiobacillus ferrivorans]
MEKLELHTWDSAEHLKTEEDIAAYLNACFEEAGDDAAFIARALGTVARARGMTRLAHDAGMGRESLYKALSGEGNPSFAT
ILKVAKALGVRIRAEALHV

Nucleotide

• Length: 300 bp

>AT168941 NZ_CP076262:1505902-1506080 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCACATTCTGATTTGGTTAGTTTTGTTTTTGAGTATCTTGTCCAGTTGGTAGGCGAACCGCCTTCCTTTT
CAATAGTGGCAGTAATTTT