Detailed information of TA system TA168935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1836434..1837471
• Replicon: chromosome
• Accession: NZ_CP059488
• Organism: Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H2515_RS09130
• Protein ID: WP_198659916.1
• Coordinates: 1836899..1837471 (+)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A7T4WBP9
• Locus tag: H2515_RS09125
• Protein ID: WP_012535904.1
• Coordinates: 1836434..1836898 (+)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2515_RS09120	1836153..1836437	+	285	WP_198659915.1	TIR domain-containing protein	-
H2515_RS09125	1836434..1836898	+	465	WP_012535904.1	helix-turn-helix domain-containing protein	Antitoxin
H2515_RS09130	1836899..1837471	+	573	WP_198659916.1	PIN domain-containing protein	Toxin
H2515_RS09135	1837595..1837753	+	159	WP_198659917.1	transposase	-
H2515_RS09140	1837826..1838266	-	441	WP_198659918.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
H2515_RS09145	1838439..1838708	+	270	WP_198659919.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
H2515_RS09150	1838769..1838969	+	201	WP_198659920.1	type II toxin-antitoxin system YafQ family toxin	-
H2515_RS09155	1839493..1840590	+	1098	WP_198659921.1	HNH endonuclease	-
H2515_RS09160	1840494..1841738	-	1245	WP_198659922.1	DNA (cytosine-5-)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1807777..1850225	42448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (83-130)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21766.99 Da
• Isoelectric Point: 6.4939

>T168935 WP_198659916.1 NZ_CP059488:1836899-1837471 [Acidithiobacillus ferrivorans]
MSSNFIVIYDACVLYPAPLRDMLMRLALTDLYRARWTDMIHDEWTRNVLKQRPDLKAEDLERTRSLMNAHVRDSLVTGFE
HLIPSVELPDADDRHVVAAAIHGGASLIVTFNLKDFPPERLKPYNLTAQHPDDFIFDLLDLHAARVCEAAANHRRSLKNP
PKTVDEYLDTLLKQGLTQTVGQLREWKVAI

Nucleotide

• Length: 573 bp

>T168935 NZ_CP076261:84385-84801 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGTGAGCAGCCGGAAGCTGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTTCAGCTGGTTGACGCATTCTGTGAGCGACTCGATGCCGTCCTGCCCTGGGATCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAAAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 16999.34 Da
• Isoelectric Point: 6.0003

>AT168935 WP_012535904.1 NZ_CP059488:1836434-1836898 [Acidithiobacillus ferrivorans]
MTTLSPTHSLPTAEEVAIARESGRALSAFLQTRAETQQIEIFNDKGASHSVRVPVSALRLLVDVLTEIGEGNAVSIIPIH
AELTTQDAADVLNVSRPFLVQLLERGEIPFHKIGTHRRVRYQDVIAYKERIDAERSKALDALAEQAQALKMGYE

Nucleotide

• Length: 465 bp

>AT168935 NZ_CP076261:84158-84388 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTATCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7T4WBP9