Detailed information of TA system TA168933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1644482..1644962
• Replicon: chromosome
• Accession: NZ_CP059488
• Organism: Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: H2515_RS08170
• Protein ID: WP_198661438.1
• Coordinates: 1644699..1644962 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: G0JS88
• Locus tag: H2515_RS08165
• Protein ID: WP_014028942.1
• Coordinates: 1644482..1644706 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2515_RS08150	1639697..1643134	+	3438	WP_198659802.1	type IV pilin biogenesis protein	-
H2515_RS08155	1643612..1643818	+	207	WP_198659803.1	hypothetical protein	-
H2515_RS08160	1643815..1643979	+	165	WP_198659804.1	DUF2283 domain-containing protein	-
H2515_RS08165	1644482..1644706	+	225	WP_014028942.1	TraY domain-containing protein	Antitoxin
H2515_RS08170	1644699..1644962	+	264	WP_198661438.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2515_RS08175	1645183..1645440	+	258	WP_014028940.1	antitoxin	-
H2515_RS08180	1645401..1645763	+	363	WP_190277971.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H2515_RS08185	1646099..1646434	-	336	WP_198659805.1	helix-turn-helix domain-containing protein	-
H2515_RS08190	1646415..1646785	-	371	Protein_1609	type II toxin-antitoxin system RelE/ParE family toxin	-
H2515_RS08195	1647098..1647313	+	216	WP_014028935.1	type II toxin-antitoxin system VapB family antitoxin	-
H2515_RS08200	1647300..1647713	+	414	WP_198659806.1	PIN domain nuclease	-
H2515_RS08205	1648078..1648299	+	222	WP_198659807.1	hypothetical protein	-
H2515_RS08210	1648361..1648639	+	279	WP_198659808.1	hypothetical protein	-
H2515_RS08215	1648780..1649142	+	363	WP_083091626.1	helix-turn-helix domain-containing protein	-
H2515_RS08220	1649142..1649627	+	486	WP_198659809.1	arsenate reductase ArsC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-85)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10031.78 Da
• Isoelectric Point: 10.4598

>T168933 WP_198661438.1 NZ_CP059488:1644699-1644962 [Acidithiobacillus ferrivorans]
MIEIDDKVKKDLAALDKAIVKRITTFLRERIAQLDDPRSIGEALKGSKLGAFWKYRVGDYRIIASIEDSALRILVIRIGN
RKEVYRQ

Nucleotide

• Length: 264 bp

>T168933 NZ_CP076260:c51836-51531 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8519.59 Da
• Isoelectric Point: 4.2910

>AT168933 WP_014028942.1 NZ_CP059488:1644482-1644706 [Acidithiobacillus ferrivorans]
MLAIRLPAEVETRLEALAQATGRTKTFYAREAILEHLDDLEDLYLAEQRLIDIRAGRSRTYTLDEVERDLGLDD

Nucleotide

• Length: 225 bp

>AT168933 NZ_CP076260:c52056-51838 [Escherichia coli]
ATGAAACAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1E7XSP6