Detailed information of TA system TA168932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1631246..1631826
• Replicon: chromosome
• Accession: NZ_CP059488
• Organism: Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: H2515_RS08080
• Protein ID: WP_198659791.1
• Coordinates: 1631500..1631826 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A060UKB1
• Locus tag: H2515_RS08075
• Protein ID: WP_035191490.1
• Coordinates: 1631246..1631503 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2515_RS08055	1627513..1628034	+	522	WP_198659788.1	AAA family ATPase	-
H2515_RS08060	1628072..1628422	+	351	WP_035191717.1	Rieske (2Fe-2S) protein	-
H2515_RS08065	1628477..1628704	-	228	WP_198659789.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H2515_RS08070	1629177..1631066	-	1890	WP_198659790.1	molecular chaperone HtpG	-
H2515_RS08075	1631246..1631503	+	258	WP_035191490.1	antitoxin	Antitoxin
H2515_RS08080	1631500..1631826	+	327	WP_198659791.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H2515_RS08085	1631849..1632250	-	402	WP_198659792.1	type II toxin-antitoxin system VapC family toxin	-
H2515_RS08090	1632247..1632498	-	252	WP_014028964.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H2515_RS08095	1632623..1632904	-	282	WP_198659793.1	type II toxin-antitoxin system ParD family antitoxin	-
H2515_RS08100	1633001..1633177	+	177	Protein_1591	type II toxin-antitoxin system YhaV family toxin	-
H2515_RS08105	1633203..1633697	-	495	WP_198659794.1	SEL1-like repeat protein	-
H2515_RS08110	1634076..1634606	+	531	WP_198659795.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
H2515_RS08115	1634830..1635321	+	492	WP_198659796.1	hypothetical protein	-
H2515_RS08120	1635342..1635554	+	213	WP_035191500.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-106)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11757.64 Da
• Isoelectric Point: 8.5317

>T168932 WP_198659791.1 NZ_CP059488:1631500-1631826 [Acidithiobacillus ferrivorans]
MRRGDIYLVSLDPTEGREQRGSRPVLVVSPAEFNEATKLPVICPITNGGDFARRIGFAVPVTGIKTTGVVRCDQPRVLDL
GARNARKVDTLPTSILEEVLAKLAPIFE

Nucleotide

• Length: 327 bp

>T168932 NZ_CP076260:6315-6713 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGCACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9196.59 Da
• Isoelectric Point: 4.6472

>AT168932 WP_035191490.1 NZ_CP059488:1631246-1631503 [Acidithiobacillus ferrivorans]
MYTTNLRKVGGSVMLVVPPAFLDQLHLQAGATVGVVVTEGRLVIDPKPRPRYTLAELLAASDYSQPQPPEEREWVDAPAV
GGELI

Nucleotide

• Length: 258 bp

>AT168932 NZ_CP076260:6088-6315 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A060UKB1