Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 866532..867208 Replicon chromosome
Accession NZ_CP059488
Organism Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)


Gene name vapC Uniprot ID G0JPF4
Locus tag H2515_RS04435 Protein ID WP_014029745.1
Coordinates 866783..867208 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A060UTH6
Locus tag H2515_RS04430 Protein ID WP_035195166.1
Coordinates 866532..866786 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H2515_RS04410 861615..862703 - 1089 WP_198660987.1 virulence RhuM family protein -
H2515_RS04415 862751..865483 - 2733 WP_198660988.1 type I restriction endonuclease subunit R -
H2515_RS04420 865550..865873 - 324 WP_014029747.1 transcriptional regulator -
H2515_RS04425 866253..866441 + 189 WP_198661404.1 BrnT family toxin -
H2515_RS04430 866532..866786 + 255 WP_035195166.1 Arc family DNA-binding protein Antitoxin
H2515_RS04435 866783..867208 + 426 WP_014029745.1 type II toxin-antitoxin system VapC family toxin Toxin
H2515_RS04440 867390..867617 - 228 WP_198660989.1 hypothetical protein -
H2515_RS04450 867989..868996 - 1008 WP_014029744.1 class I SAM-dependent methyltransferase -
H2515_RS04455 869012..869773 - 762 WP_198660990.1 sulfite exporter TauE/SafE family protein -
H2515_RS04460 869940..870893 + 954 WP_198660991.1 D-alanyl-D-alanine carboxypeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

Arc-like DNA binding domain

(9-46)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15189.80 Da        Isoelectric Point: 7.1661

>T168928 WP_014029745.1 NZ_CP059488:866783-867208 [Acidithiobacillus ferrivorans]
MILLDTNVISEPLRKAPEICVVTWIDAQPIETLYLSTVTIAELRLGIAMLPTGRRRDILHENLEDRVLPMFVGRVLAFDL
AASQAFAHLMAKSRGAGFAIGRADGYIAAIAAANKMTVATRDTGPFLSAGITVINPWALSA

Download         Length: 426 bp

>T168928 NZ_CP076259:4270841-4271152 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9627.08 Da        Isoelectric Point: 5.9068

>AT168928 WP_035195166.1 NZ_CP059488:866532-866786 [Acidithiobacillus ferrivorans]
MAMLTIRNLPEEVRRALRMRAAQHGRSTEAEVRSILEEVVKPQGRVKLGSLLTEIGRRADLTDEDLAVFERVRDKTPEEP
VLFE

Download         Length: 255 bp

>AT168928 NZ_CP076259:4271153-4271443 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060UUA7

Antitoxin

Source ID Structure
AlphaFold DB A0A060UTH6

References