Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1060..1607 Replicon plasmid pIB2020_N
Accession NZ_CP059485
Organism Enterobacter kobei strain IB2020

Toxin (Protein)


Gene name ccdB Uniprot ID A0A2W0G5G9
Locus tag H1R18_RS26475 Protein ID WP_037427062.1
Coordinates 1060..1368 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A663ALK6
Locus tag H1R18_RS26480 Protein ID WP_064179603.1
Coordinates 1368..1607 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R18_RS26460 1..6127 - 6127 WP_182115642.1 hypothetical protein -
H1R18_RS26465 174..659 + 486 WP_139156959.1 MbeB family mobilization protein -
H1R18_RS26470 720..956 + 237 WP_037427059.1 MbeD family mobilization/exclusion protein -
H1R18_RS26475 1060..1368 - 309 WP_037427062.1 CcdB family protein Toxin
H1R18_RS26480 1368..1607 - 240 WP_064179603.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H1R18_RS26485 1889..2812 + 924 WP_098947601.1 replication initiation protein -
H1R18_RS26490 2812..3285 + 474 WP_104652423.1 hypothetical protein -
H1R18_RS26495 3316..3789 + 474 WP_084797509.1 hypothetical protein -
H1R18_RS26500 4003..4983 - 981 WP_213014372.1 IS5-like element IS5 family transposase -
H1R18_RS26505 5297..5632 + 336 WP_037427024.1 plasmid mobilization relaxosome protein MobC -
H1R18_RS26510 5577..6095 + 519 Protein_10 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..6127 6127
- flank IS/Tn - - 4003..4983 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(9-79)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11792.44 Da        Isoelectric Point: 4.6135

>T168923 WP_037427062.1 NZ_CP059485:c1368-1060 [Enterobacter kobei]
MQFMVYRNNSNNRAYPYLLDVQSDIIDELNTRLVIPLFLLDEVNGRPARRLTPVLNVDGEDFLVMTHEMASVRQSQLGEE
VVSVREHRQAIKNALDFLLDGF

Download         Length: 309 bp

>T168923 NZ_CP076259:c3054618-3054283 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9025.29 Da        Isoelectric Point: 10.5889

>AT168923 WP_064179603.1 NZ_CP059485:c1607-1368 [Enterobacter kobei]
MPVSAMRHKKTVSVTIEPALLHQAREAGINLSATLTTALKHQIRESEAERWKRANRKAIQELNRITDEHGLLSDDHRTF

Download         Length: 240 bp

>AT168923 NZ_CP076259:c3054866-3054618 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G5G9

Antitoxin

Source ID Structure
AlphaFold DB A0A663ALK6

References