Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 52081..52817 Replicon plasmid pIB2020_L
Accession NZ_CP059484
Organism Enterobacter kobei strain IB2020

Toxin (Protein)


Gene name tacT Uniprot ID A0A0J1M830
Locus tag H1R18_RS25910 Protein ID WP_048242169.1
Coordinates 52335..52817 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A0J1M9H0
Locus tag H1R18_RS25905 Protein ID WP_048242172.1
Coordinates 52081..52347 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R18_RS25880 47756..47956 + 201 WP_048242179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
H1R18_RS25885 48273..49289 + 1017 WP_048242177.1 replication initiation protein -
H1R18_RS25890 50427..50834 + 408 WP_182115636.1 hypothetical protein -
H1R18_RS25895 50870..51435 + 566 Protein_56 DUF2913 family protein -
H1R18_RS25900 51451..51717 + 267 WP_048242174.1 hypothetical protein -
H1R18_RS25905 52081..52347 + 267 WP_048242172.1 DUF1778 domain-containing protein Antitoxin
H1R18_RS25910 52335..52817 + 483 WP_048242169.1 GNAT family N-acetyltransferase Toxin
H1R18_RS25915 52884..53234 + 351 WP_048242167.1 hypothetical protein -
H1R18_RS25920 53746..54240 - 495 WP_048242271.1 thermonuclease family protein -
H1R18_RS25925 54240..55232 - 993 WP_048242165.1 hypothetical protein -
H1R18_RS25930 55544..57571 - 2028 WP_048242163.1 acyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..150133 150133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-150)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17394.03 Da        Isoelectric Point: 9.8047

>T168922 WP_048242169.1 NZ_CP059484:52335-52817 [Enterobacter kobei]
VGRVTAPEPLSSSHQLAEFVSGESVLDEWLKQRGLKNQALGAARTFVVCKSGTKQVAGFYSLATGSVNHTESTGSLRRNM
PDPIPVIILARLAVDVAFRGKGIGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYLYHGFKASNTQERTLFLKLP

Download         Length: 483 bp

>T168922 NZ_CP076259:c2731845-2731447 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT168922 WP_048242172.1 NZ_CP059484:52081-52347 [Enterobacter kobei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIAMLDEPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT168922 NZ_CP076259:c2732072-2731845 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1M830

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1M9H0

References