Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3125419..3126216 Replicon chromosome
Accession NZ_CP059481
Organism Enterobacter kobei strain IB2020

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag H1R18_RS15995 Protein ID WP_173674980.1
Coordinates 3125419..3125943 (-) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A2W0G3H3
Locus tag H1R18_RS16000 Protein ID WP_014883303.1
Coordinates 3125947..3126216 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R18_RS15965 3121002..3121691 + 690 WP_014883310.1 pyrimidine utilization protein B -
H1R18_RS15970 3121703..3122089 + 387 WP_014883309.1 pyrimidine utilization protein C -
H1R18_RS15975 3122097..3122897 + 801 WP_014883308.1 pyrimidine utilization protein D -
H1R18_RS15980 3122907..3123497 + 591 WP_014883307.1 malonic semialdehyde reductase -
H1R18_RS15985 3123507..3124001 + 495 WP_023332659.1 pyrimidine utilization flavin reductase protein F -
H1R18_RS15990 3124023..3125345 + 1323 WP_014883305.1 pyrimidine utilization transport protein G -
H1R18_RS15995 3125419..3125943 - 525 WP_173674980.1 GNAT family N-acetyltransferase Toxin
H1R18_RS16000 3125947..3126216 - 270 WP_014883303.1 DUF1778 domain-containing protein Antitoxin
H1R18_RS16005 3126278..3127195 - 918 WP_047028038.1 DMT family transporter -
H1R18_RS16010 3127312..3127482 - 171 WP_023332656.1 general stress protein -
H1R18_RS16015 3127874..3128470 + 597 WP_014883301.1 NAD(P)H:quinone oxidoreductase -
H1R18_RS16020 3128489..3128716 + 228 WP_014883300.1 YccJ family protein -
H1R18_RS16025 3128750..3129991 - 1242 WP_023337066.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
H1R18_RS16030 3130095..3130595 - 501 WP_014883299.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(90-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19662.52 Da        Isoelectric Point: 8.4738

>T168915 WP_173674980.1 NZ_CP059481:c3125943-3125419 [Enterobacter kobei]
IVANLTIEMFSEGSDYDFRGFDCGEPSLNAFLTEHLVRQHKGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSTTQQ
RRIPYVNVPSVTLGRLAVHKDLQGKEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAKRFYLKQGFIPLTGENSHSL
FFPTKSIEQLFEQA

Download         Length: 525 bp

>T168915 NZ_CP076259:c1368363-1368256 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10278.72 Da        Isoelectric Point: 5.8836

>AT168915 WP_014883303.1 NZ_CP059481:c3126216-3125947 [Enterobacter kobei]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNESSWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT168915 NZ_CP076259:1368410-1368476 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0G3H3

References