Detailed information of TA system TA168899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 20077..20661
• Replicon: plasmid p17-84_OXA
• Accession: NZ_CP059478
• Organism: Acinetobacter baumannii strain 17-84

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: H2Q98_RS00125
• Protein ID: WP_000286964.1
• Coordinates: 20077..20379 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H2Q98_RS00130
• Protein ID: WP_000985609.1
• Coordinates: 20380..20661 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2Q98_RS00080	15374..16573	+	1200	WP_002072939.1	hypothetical protein	-
H2Q98_RS00085	16646..16888	-	243	WP_001015364.1	hypothetical protein	-
H2Q98_RS00090	17037..17261	+	225	WP_002072934.1	hypothetical protein	-
H2Q98_RS00095	17365..17568	+	204	WP_182923962.1	hypothetical protein	-
H2Q98_RS00100	17573..17848	+	276	WP_005140357.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
H2Q98_RS00105	17832..18122	+	291	WP_000176412.1	type II toxin-antitoxin system YafQ family toxin	-
H2Q98_RS00110	18170..18316	-	147	WP_000192351.1	hypothetical protein	-
H2Q98_RS00115	18313..18834	-	522	WP_000999915.1	hypothetical protein	-
H2Q98_RS00120	19398..19928	-	531	WP_000172759.1	hypothetical protein	-
H2Q98_RS00125	20077..20379	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2Q98_RS00130	20380..20661	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
H2Q98_RS00135	20741..21160	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
H2Q98_RS00140	21300..21620	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H2Q98_RS00145	21613..21885	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
H2Q98_RS00150	22378..23853	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
H2Q98_RS00155	23909..24793	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58 / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / blaPER-1 / aph(3')-VIb / sul2 / aac(3)-IId	-	1..108715	108715
	-	flank	IS/Tn	msr(E) / mph(E)	-	22378..25806	3428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T168899 WP_000286964.1 NZ_CP059478:20077-20379 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T168899 NZ_CP076255:c1149574-1149317 [Xanthomonas translucens pv. graminis]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATCCAACGCGATCCCTTCCAAGGCGTCGGCAAGCCAGAACCGCTGCGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTGTACAAGGTCGAGGCGGGCATCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT168899 WP_000985609.1 NZ_CP059478:20380-20661 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT168899 NZ_CP076255:c1149822-1149571 [Xanthomonas translucens pv. graminis]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTCGCGGACACCATGGAGCGTGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAT
CTGGCCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94