Detailed information of TA system TA168897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 9541..10129
• Replicon: plasmid p17-84_OXA
• Accession: NZ_CP059478
• Organism: Acinetobacter baumannii strain 17-84

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: H2Q98_RS00045
• Protein ID: WP_000438825.1
• Coordinates: 9541..9828 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: H2Q98_RS00050
• Protein ID: WP_004728120.1
• Coordinates: 9815..10129 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2Q98_RS00020	5567..5935	-	369	Protein_3	IS1-like element ISAba3 family transposase	-
H2Q98_RS00025	6016..6858	+	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
H2Q98_RS00030	6949..7701	+	753	Protein_5	IS1-like element ISAba3 family transposase	-
H2Q98_RS00035	7829..8656	+	828	WP_000073658.1	AraC family transcriptional regulator	-
H2Q98_RS00040	8706..9311	+	606	WP_000447788.1	LysE family translocator	-
H2Q98_RS00045	9541..9828	+	288	WP_000438825.1	BrnT family toxin	Toxin
H2Q98_RS00050	9815..10129	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
H2Q98_RS00055	10179..10496	-	318	WP_005065053.1	hypothetical protein	-
H2Q98_RS00060	10847..11413	-	567	WP_000858429.1	plasmid replication DNA-binding protein	-
H2Q98_RS00065	11477..12406	-	930	WP_000200914.1	replication initiation protein RepM	-
H2Q98_RS00070	12906..14066	-	1161	WP_000179382.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58 / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / blaPER-1 / aph(3')-VIb / sul2 / aac(3)-IId	-	1..108715	108715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T168897 WP_000438825.1 NZ_CP059478:9541-9828 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T168897 NZ_CP076255:256660-256812 [Xanthomonas translucens pv. graminis]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTCTGTGCAGGCATGCTGACTGGCTGCAACACCATGGCCGGTGC
TGGCAAGGATATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGCAAAGACGGCAAGTGCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT168897 WP_004728120.1 NZ_CP059478:9815-10129 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT168897 NZ_CP076255:256912-257046 [Xanthomonas translucens pv. graminis]
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTTTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9