Detailed information of TA system TA168893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 58670..59406
• Replicon: plasmid pIncFIBK_FIIK
• Accession: NZ_CP059470
• Organism: Klebsiella pneumoniae strain BA25665

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: HBJ46_RS28150
• Protein ID: WP_003026803.1
• Coordinates: 58924..59406 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HBJ46_RS28145
• Protein ID: WP_003026799.1
• Coordinates: 58670..58936 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ46_RS28100 (HBJ46_027980)	54732..55094	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
HBJ46_RS28105 (HBJ46_027985)	55144..55494	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HBJ46_RS28110 (HBJ46_027990)	55852..56121	+	270	WP_004152102.1	hypothetical protein	-
HBJ46_RS28115 (HBJ46_027995)	56109..56684	+	576	WP_004152103.1	hypothetical protein	-
HBJ46_RS28120 (HBJ46_028000)	56715..57209	+	495	WP_004152104.1	DNA-binding protein	-
HBJ46_RS28125 (HBJ46_028005)	57253..57621	+	369	WP_004152105.1	hypothetical protein	-
HBJ46_RS28130 (HBJ46_028010)	57655..57858	+	204	WP_004152106.1	HHA domain-containing protein	-
HBJ46_RS28135 (HBJ46_028015)	57907..58164	+	258	WP_004152107.1	hypothetical protein	-
HBJ46_RS28140 (HBJ46_028020)	58240..58494	+	255	WP_004152108.1	hypothetical protein	-
HBJ46_RS28145 (HBJ46_028025)	58670..58936	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HBJ46_RS28150 (HBJ46_028030)	58924..59406	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
HBJ46_RS28155 (HBJ46_028035)	59618..60964	+	1347	WP_020314316.1	ISNCY family transposase	-
HBJ46_RS28965	61125..61256	+	132	WP_004218042.1	hypothetical protein	-
HBJ46_RS28160 (HBJ46_028040)	61465..62630	-	1166	Protein_67	IS3 family transposase	-
HBJ46_RS28165 (HBJ46_028045)	62807..63769	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
HBJ46_RS28170 (HBJ46_028050)	63756..64244	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1	-	1..211813	211813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T168893 WP_003026803.1 NZ_CP059470:58924-59406 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T168893 NZ_CP076254:c221147-221013 [Xanthomonas translucens pv. graminis]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTTTCGGTAGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT168893 WP_003026799.1 NZ_CP059470:58670-58936 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT168893 NZ_CP076254:c221398-221246 [Xanthomonas translucens pv. graminis]
ATGAAGCGACTGTTCACCTTGATGATGCTGACCCTGTTCTGTGCAGGCATGCTGACTGGCTGCAACACCATGGCCGGTGC
TGGCAAGGATATGCAGAAGGCCGGCGATAAGGTGGAAAACAAGGCCGACGACTGCAAAGACGGCAAGTGCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66