Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 167689..168599 Replicon plasmid pIncHI1B_FIB
Accession NZ_CP059469
Organism Klebsiella pneumoniae strain BA25665

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag HBJ46_RS27210 Protein ID WP_004181896.1
Coordinates 168129..168599 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag HBJ46_RS27205 Protein ID WP_004181895.1
Coordinates 167689..168132 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ46_RS27185 (HBJ46_027065) 162966..163673 + 708 WP_062826779.1 hypothetical protein -
HBJ46_RS27190 (HBJ46_027070) 163678..164709 + 1032 WP_170856552.1 CRISPR-associated protein Csf2 -
HBJ46_RS27195 (HBJ46_027075) 165895..166317 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HBJ46_RS27200 (HBJ46_027080) 166317..167588 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
HBJ46_RS27205 (HBJ46_027085) 167689..168132 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
HBJ46_RS27210 (HBJ46_027090) 168129..168599 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
HBJ46_RS27215 (HBJ46_027095) 168709..168969 - 261 WP_004026352.1 hypothetical protein -
HBJ46_RS27220 (HBJ46_027100) 169540..170721 - 1182 WP_004883380.1 IS481 family transposase -
HBJ46_RS27225 (HBJ46_027105) 170793..170951 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
HBJ46_RS27230 (HBJ46_027110) 171554..172528 + 975 WP_043907013.1 hypothetical protein -
HBJ46_RS27235 (HBJ46_027115) 172726..173103 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 - 1..289419 289419
- flank IS/Tn - - 169540..170721 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T168891 WP_004181896.1 NZ_CP059469:168129-168599 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T168891 NZ_CP076253:c4088245-4088111 [Xanthomonas translucens pv. graminis]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTTTCGGTAGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT168891 WP_004181895.1 NZ_CP059469:167689-168132 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT168891 NZ_CP076253:c4088496-4088344 [Xanthomonas translucens pv. graminis]
ATGAAGCGACTGTTCACCTTGATGATGCTGACCCTGTTCTGTGCAGGCATGCTGACTGGCTGCAACACCATGGCCGGTGC
TGGCAAGGATATGCAGAAGGCCGGCGATAAGGTGGAAAACAAGGCCGACGACTGCAAAGACGGCAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References